SGK1 Polyclonal Antibody

SKU:BHA12109466
Overview
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SGK1-specific polyclonal antibody raised in Rabbit. Validated for Western Blot, ELISA in Human samples. Affinity-purified rabbit IgG.
Specificity Total Protein
Immunogen Synthetic Peptide
Molecular Weight ~48.9 kDa
UniProt O00141
Gene ID Human: 6446
Applications 2 validated
Options selector
Catalog no. Size
BT-AP08256-20UL 20 uL
BT-AP08256-50UL 50 uL
BT-AP08256-100UL 100 uL
Available Options
Size
Field Specification
Mfr No BT-AP08256
Alternative Names SGK1; SGK; Serine/threonine-protein kinase Sgk1; Serum/glucocorticoid-regulated kinase 1
Clonality
  • Polyclonal
Concentration 1 mg/ml
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Gene ID Human: 6446
Host Rabbit
Immunogen Synthesized peptide derived from SGK1 . at AA range: 180-260
Isotype
  • IgG
Molecular Weight ~48.9 kDa
Product Type
  • Primary Antibody
Reactivity
  • Human
Storage -20°C for 1 year
Target SGK1

Product Overview

Primary Antibody

This antibody targets SGK1 and detects endogenous levels of SGK1 protein.

Validated Applications

Application Dilution Guidance
Western Blot (WB) Refer to datasheet for recommended working dilution
ELISA Refer to datasheet for recommended working dilution
✔ Validated by Bioassay Technology Laboratory

Antibody Specifications

Target SGK1
Host Rabbit
Clonality Polyclonal
Isotype IgG
Reactivity Human
Purification Affinity Purification
Molecular Weight ~48.9 kDa
Concentration 1 mg/ml
Immunogen Synthesized peptide derived from SGK1 . at AA range: 180-260
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage -20°C for 1 year
Gene ID Human: 6446
UniProt O00141 (Human: O00141; Mouse: Q9WVC6)

Scientific Background

SGK1 (serum/glucocorticoid regulated kinase 1) encodes a serine/threonine protein kinase that plays an important role in cellular stress response. This kinase activates certain potassium, sodium, and chloride channels, suggesting an involvement in the regulation of processes such as cell survival, neuronal excitability, and renal sodium excretion. High levels of expression of SGK1 may contribute to conditions such as hypertension and diabetic nephropathy. Several alternatively spliced transcript variants encoding different isoforms have been noted for SGK1.

Alternative Names

SGK1; SGK; Serine/threonine-protein kinase Sgk1; Serum/glucocorticoid-regulated kinase 1

Safety & Handling

⚠ Research Use Only (RUO). Not approved for diagnostic or therapeutic use. Handle with standard laboratory safety precautions. Contains sodium azide — avoid contact with skin/eyes; dispose per local regulations.
TargetSGK1
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ReactivityHuman
PurificationAffinity Purification
Molecular Weight~48.9 kDa
Concentration1 mg/ml
ImmunogenSynthesized peptide derived from SGK1 . at AA range: 180-260
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage-20°C for 1 year
Gene IDHuman: 6446
UniProtO00141 (Human: O00141; Mouse: Q9WVC6)

Optimized working dilutions depend on sample type and detection system. Titration is recommended.

ApplicationDilution Note
Western Blot (WB)Refer to datasheet for recommended working dilution
ELISARefer to datasheet for recommended working dilution

SGK1 (serum/glucocorticoid regulated kinase 1) encodes a serine/threonine protein kinase that plays an important role in cellular stress response. This kinase activates certain potassium, sodium, and chloride channels, suggesting an involvement in the regulation of processes such as cell survival, neuronal excitability, and renal sodium excretion. High levels of expression of SGK1 may contribute to conditions such as hypertension and diabetic nephropathy. Several alternatively spliced transcript variants encoding different isoforms have been noted for SGK1.

Alternative Names

SGK1; SGK; Serine/threonine-protein kinase Sgk1; Serum/glucocorticoid-regulated kinase 1

What species does this antibody react with?
This antibody has been validated for reactivity against Human. Actual cross-reactivity is determined by immunogen sequence homology across species. Contact technical support for unlisted species.
What applications has this antibody been validated for?
Validated applications include: Western Blot (WB), ELISA. Each application was tested with positive and negative controls. Contact our team regarding applications not listed.
How was this antibody produced and purified?
This polyclonal IgG antibody was raised in rabbit against a synthesized target-specific peptide. It was affinity-purified from antiserum using an epitope-specific immunogen, yielding a highly specific antibody suitable for multiple research applications.
What is the expected molecular weight of SGK1?
The predicted molecular weight of SGK1 is ~48.9 kDa. Band size may vary slightly depending on post-translational modifications and gel percentage.
What storage conditions does this antibody require?
Store at −20°C for long-term storage. Avoid repeated freeze-thaw cycles. Working aliquots can be stored at 4°C for short-term use. The formulation includes 50% glycerol as a cryoprotectant.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.

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