| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | SGOL2; Shugoshin-like 2; Shugoshin-2; Sgo2; Tripin |
| Clonality | |
| Concentration | |
| Gene ID | |
| Host | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human SGOL2. AA range:681-730 |
| Isotype | |
| Product Type | |
| Reactivity | |
| Storage |
Product Overview
function:During meiosis, protects centromeric cohesion complexes until metaphase II/anaphase II transition, preventing premature release of meiosis-specific REC8 cohesin complexes from anaphase I centromeres. Is thus essential for an accurate gametogenesis (By similarity). May act by targeting PPP2CA to centromeres, thus leading to cohesin dephosphorylation.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,sequence caution:Contaminating sequence. Potential poly-A sequence.,similarity:Belongs to the shugoshin family.,subcellular location:In Hela cells, localizes at centromeres throughout prophase until metaphase and disappears at anaphase.,subunit:Directly interacts with PPP2CA.,
Polyclonal Rabbit AntibodyValidated Applications
| Application | Recommended Dilution |
|---|---|
| Immunohistochemistry | Refer to datasheet |
| Immunofluorescence | Refer to datasheet |
| ELISA | Refer to datasheet |
Immunogen & Specificity
Immunogen: The antiserum was produced against synthesized peptide derived from human SGOL2. AA range:681-730
Subcellular Localization: Nucleus . Chromosome, centromere . Chromosome, centromere, kinetochore . During meiosis I, accumulates at centromeres during diplotene, and colocalizes differentially with the cohesin subunits RAD21 and REC8 at metaphase I centromeres (By similarity). SGO2 and RAD21 change their relative distributions during telophase I when sister-kinetochore association is lost (By similarity). During meiosis II, it shows a striking tension-dependent redistribution within centromeres throughout chromosome congression during prometaphase II, as it does during mitosis (By similarity). In Hela cells, localizes at centromeres throughout prophase until metaphase and disappears at anaphase (PubMed:17485487). Centromeric localization requires the presence of BUB1 and AUKRB (PubMed:17485487). .
UniProt/Swiss-Prot: Q562F6
Product Specifications
| Host Species | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | IgG |
| Species Reactivity | Human, Rat, Mouse |
| Concentration | 1 mg/ml |
| Storage | -20°C/1 year |
Reactivity Notes
Confirmed reactivity in Human, Rat, Mouse samples; validated for Immunohistochemistry, Immunofluorescence, ELISA.
Safety & Handling
- For Research Use Only (RUO). Not intended for diagnostic procedures.
- Shipping: cold-chain shipment (typically with ice packs).
- Upon receipt: store at recommended temperature as soon as possible.
- Sales terms and conditions: Please review prior to ordering.
Related Products
Explore additional antibodies and related detection reagents for Sgo2 in the BioHippo catalog.
This antibody has been validated for Immunohistochemistry, Immunofluorescence, ELISA. Refer to the product datasheet for recommended dilutions and protocol guidance for each application.
Refer to the datasheet for recommended dilution ranges per application. Optimal dilution may vary by sample type, fixation method, and detection system — a titration experiment is recommended prior to full experimental use.
This antibody has been validated or predicted to recognize Human, Rat, Mouse samples, based on immunogen sequence homology. For unlisted species, contact our team for guidance.
This antibody was raised against a specific peptide or recombinant protein fragment corresponding to Sgo2. Exact immunogen details are provided in the product datasheet.
Conjugated formats may be available upon request. Contact the BioHippo team for custom conjugation options including fluorophore, biotin, or enzyme labeling.
BioHippo can support custom antibody formatting and service options for selected products. Contact us to discuss your project requirements.
Potential customization options include:
- Custom conjugation: fluorophore, biotin, enzyme, or nanoparticle labeling available on request for qualified antibodies.
- Bulk or scale-up quantities: volume pricing and custom lead time estimates available for larger orders.
- Alternative formulations: azide-free, carrier protein-free, or lyophilized formats available upon request for selected products.
- OEM and private-label supply: custom packaging and branding available for commercial applications.
Submit your project specifications via the product contact form or reach us at support@ebiohippo.com for a tailored quotation.
