| Field | Specification |
|---|---|
| CAS no. | |
| Applications | |
| Molecular weight | |
| Molecular formula | C19H32ClNO |
| Purity | |
| SMILES | |
| Form | Liquid |
| Storage | |
| Shipping | |
| Catalog no. (Mfr.) | |
| Main SKU |
Compound Overview
SH-BC-893 is an orally active anti-neoplastic sphingolipid analog that protects against ceramide-induced mitochondrial dysfunction and corrects diet-induced obesity, and it can be used for research on cancer and obesity[1][2]. It is supplied as a colorless to light yellow liquid (C19H32ClNO, MW 325.92) at 97.31% purity.
Physical & Chemical Properties
| CAS Number | 1841409-92-4 |
|---|---|
| Molecular Formula | C19H32ClNO |
| Molecular Weight | 325.92 g/mol |
| Purity | 97.31% |
| Appearance | Liquid |
| Color | Colorless to light yellow |
| SMILES | OC[C@H]1NCC[C@@H]1C2=CC=C(CCCCCCCC)C=C2.Cl |
| Signaling Pathway | Metabolic Enzyme/Protease |
| Solubility | In Vitro: DMSO: 50 mg/mL (153.41 mM; Requires sonication; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO) |
| Storage | -20°C, sealed storage, away from moisture. In solvent: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). |
| Shipping | Room temperature in continental US; may vary elsewhere. |
Literature Cited
Sources cited in this description and in the In Vitro & In Vivo Data tab. Peer-reviewed publications that used this product are listed under References.
Safety
For Research Use Only. Not for use in diagnostic or therapeutic procedures, and not for human or veterinary use. Handle in accordance with the Safety Data Sheet and your institution's chemical hygiene plan.
In Vitro
| Solvent | Solubility | Notes |
|---|---|---|
| DMSO | 50 mg/mL (153.41 mM) | requires sonication; use freshly opened DMSO (absorbed moisture lowers solubility) |
Aliquot the stock solution and store it at -80°C (up to 6 months) or -20°C (up to 1 month); sealed storage, away from moisture; avoid repeated freeze-thaw cycles.
In Vivo
Choose the formulation that suits the animal model and route of administration; percentages are volume ratios of the final working solution. Start from a clear DMSO stock (see In Vitro above), add the co-solvents one at a time in the order listed, mixing after each addition, and prepare the working solution fresh on the day of dosing. If precipitation or phase separation occurs, gentle warming or sonication can help.
Protocol 1
| Composition | 10% DMSO + 40% PEG300 + 5% Tween-80 + 45% saline |
|---|---|
| Result | ≥ 2.5 mg/mL (7.67 mM); clear solution |
| How to prepare | Gives a clear solution at ≥ 2.5 mg/mL (saturation not determined). For 1 mL of working solution: add 100 μL DMSO stock (25.0 mg/mL) to 400 μL PEG300; then 50 μL Tween-80; then 450 μL saline to bring the volume to 1 mL. Saline: dissolve 0.9 g sodium chloride in ddH2O and make up to 100 mL. |
Protocol 2
| Composition | 10% DMSO + 90% (20% SBE-β-CD in saline) |
|---|---|
| Result | ≥ 2.5 mg/mL (7.67 mM); clear solution |
| How to prepare | Gives a clear solution at ≥ 2.5 mg/mL (saturation not determined). For 1 mL of working solution: add 100 μL DMSO stock (25.0 mg/mL) to 900 μL 20% SBE-β-CD in saline. 20% SBE-β-CD in saline: dissolve 2 g SBE-β-CD powder in 10 mL saline until clear (4°C, store up to one week). |
Protocol 3
| Composition | 10% DMSO + 90% Corn Oil |
|---|---|
| Result | ≥ 2.5 mg/mL (7.67 mM); clear solution |
| How to prepare | Gives a clear solution at ≥ 2.5 mg/mL (saturation not determined). Use with caution if continuous dosing will exceed two weeks. For 1 mL of working solution: add 100 μL DMSO stock (25.0 mg/mL) to 900 μL corn oil. |
Data provided by the manufacturer.
In Vitro
By down-regulating cell surface nutrient transporters and blocking lysosomal trafficking events, SH-BC-893 starves cancer cells to death[1]. Through disruption of intracellular trafficking, SH-BC-893 (5 μM; 3 h) protects against ceramide-induced mitochondrial network fragmentation[2]. SH-BC-893 (8.935 nM; 3 h) protects against ceramide-induced mitochondrial dysfunction[2].
Western Blot Analysis[1]
| Cell Line | p53 flox/flox MEFs |
|---|---|
| Concentration | 5 μM |
| Incubation Time | 3 h |
| Result | Blocked palmitate-induced recruitment of DRP1 to mitochondria without affecting DRP1 protein levels. |
Immunofluorescence[1]
| Cell Line | p53 flox/flox MEFs |
|---|---|
| Concentration | 5 μM |
| Incubation Time | 3 h |
| Result | Blocked palmitate-induced recruitment of DRP1 to mitochondria. |
In Vivo
A single oral 120 mg/kg dose of SH-BC-893 acutely and robustly blocks ceramide-induced mitochondrial dysfunction, and corrects diet-induced obesity along with its metabolic sequelae[1].
| Animal Model | C57BL/6J mice (Male)[1] |
|---|---|
| Dosage | 120 mg/kg |
| Administration | Oral; single |
| Result | Blocked palmitate- and ceramide-induced mitochondrial fission, preserved mitochondrial function, and prevented ER stress. Normalized mitochondrial morphology in the livers and brains of HFD-fed mice, improved mitochondrial function in white adipose tissue, and corrected aberrant plasma leptin and adiponectin levels. Restored normal body weight, glucose disposal, and hepatic lipid levels in mice consuming a HFD. |
Data provided by the manufacturer. Numbered citations refer to the Literature Cited list in the product description.
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