| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | SH3 and PX domain-containing protein 2A|Adapter protein TKS5|Five SH3 domain-containing protein|SH3 multiple domains protein 1|Tyrosine kinase substrate with five SH3 domains|SH3PXD2A|FISH|KIAA0418|SH3MD1|TKS5 |
| Clonality | |
| Form | Liquid |
| Gene ID | |
| Host | |
| Immunogen | SH3 and PX domains 2A |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Product Overview
Adapter protein involved in invadopodia and podosome formation, extracellular matrix degradation and invasiveness of some cancer cells. Binds matrix metalloproteinases(ADAMs), NADPH oxidases(NOXs) and phosphoinositides. Acts as an organizer protein that allows NOX1-or NOX3-dependent reactive oxygen species(ROS) generation and ROS localization. In association with ADAM12, mediates the neurotoxic effect of beta-amyloid peptide.
Polyclonal Rabbit AntibodyValidated Applications
| Application | Recommended Dilution |
|---|---|
| ELISA | Refer to datasheet |
| Western Blot | Refer to datasheet |
| Immunohistochemistry | Refer to datasheet |
| Immunofluorescence | Refer to datasheet |
Immunogen & Specificity
Immunogen: SH3 and PX domains 2A
UniProt/Swiss-Prot: Q5TCZ1
Product Specifications
| Host Species | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | IgG |
| Species Reactivity | Human, Mouse, Rat |
| Purification Method | Immunogen Affinity Purified |
| Purity | ≥95% as determined by SDS-PAGE |
| Molecular Weight | 140 kDa |
| Storage | PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.) |
Reactivity Notes
Confirmed reactivity in Human, Mouse, Rat samples; validated for ELISA, Western Blot, Immunohistochemistry, Immunofluorescence.
Safety & Handling
- For Research Use Only (RUO). Not intended for diagnostic procedures.
- Shipping: cold-chain shipment (typically with ice packs).
- Upon receipt: store at recommended temperature as soon as possible.
- Sales terms and conditions: Please review prior to ordering.
Related Products
Explore additional antibodies and related detection reagents for SH3PXD2A in the BioHippo catalog.
SH3PXD2A antibody
First, usually it's not suggested to recycle antibodies. After use, buffer system of antibodies has changed. The storage condition of recycled antibodies for different customers also varies. Thus, the performance efficiency of recycled antibodies can’t be guaranteed. Besides, FineTest ever conducted the antibody recycling assay. Assay results show recycling times of different antibodies also varies. Usually, higher antibody titer allows more repeated use. Customers can determine based on experimental requirements.
Notes: After incubation, we recycle rest antibodies to centrifuge tube and store at 4℃. High titer antibodies can be stored for a minimum of one week. Reuse about three times.
Components of FineTest antibody buffer are usually PBS with proclin300 or sodium azide, BSA, 50% glycerol. Common preservative is proclin300 or sodium azide, which is widely applied in the lab and industry.
Most antibodies are stored at -20℃. Directly-labeled flow cytometry antibodies should be stored at 2 - 8℃. The shelf life is one year. If after sales issues for purchased antibodies appear, return or replacement is available. Usually, antibodies can be still used after the one-year warranty. We can offer technical support services.
Directly-labeled flow cytometry antibodies are ready-to-use without dilution. Other antibodies are usually concentrated. Follow the dilution ratio suggested in the manual. Dilute solution for different experiments also varies. Common antibody dilution buffers are acceptable(e.g. PBST, TBST, antibody blocking buffer).
Common retrieval buffers: Tris-EDTA Buffer(pH 9.0); Citrate Buffer(pH 6.0)
Heat induced antibody retrieval:
Method 1: Water-bath heating: Put the beaker with retrieval buffer and slide in the boiling water bath. Keep the boiling state for 15min. Naturally cool to room temperature;
Method 2: Microwave retrieval: Put the beaker with retrieval buffer and slide in the microwave oven. Heat at high power for 5min, Switch OFF for 3min, Heat at medium power for 5min. Naturally cool to room temperature.
BioHippo can support custom antibody formatting and service options for selected products. Contact us to discuss your project requirements.
Potential customization options include:
- Custom conjugation: fluorophore, biotin, enzyme, or nanoparticle labeling available on request for qualified antibodies.
- Bulk or scale-up quantities: volume pricing and custom lead time estimates available for larger orders.
- Alternative formulations: azide-free, carrier protein-free, or lyophilized formats available upon request for selected products.
- OEM and private-label supply: custom packaging and branding available for commercial applications.
Submit your project specifications via the product contact form or reach us at support@ebiohippo.com for a tailored quotation.
