| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | SHARPIN;SIPL1;sharpin |
| Conjugate | |
| Formulation | |
| Host | |
| Immunogen | Recombinant fusion protein of human SHARPIN(NP_112236.3). |
| Molecular Weight | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage |
Product Overview
Research Use OnlyComponent of the LUBAC complex which conjugates linear polyubiquitin chains in a head-to-tail manner to substrates and plays a key role in NF-kappa-B activation and regulation of inflammation. LUBAC conjugates linear polyubiquitin to IKBKG and RIPK1 and is involved in activation of the canonical NF-kappa-B and the JNK signaling pathways. Linear ubiquitination mediated by the LUBAC complex interferes with TNF-induced cell death and thereby prevents inflammation.
Validated Applications
| Western Blot (WB) | 500 - 1:2000|IF/ICC |
|---|---|
| Immunofluorescence (IF/ICC) | 50 - 1:100 |
Key Specifications
| Host Species | Rabbit |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Conjugate | Unconjugated |
| Purification | Affinity Purification |
| Formulation | 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 |
| Molecular Weight | ~ 43 kDa |
| Catalog No. | BS71652 |
| UniProt | Q9H0F6 |
| Alternative Names | SHARPIN;SIPL1;sharpin |
Immunogen / Antigen
Recombinant fusion protein of human SHARPIN(NP_112236.3).
Reactivity Notes
Polyclonal Antibodies
Storage & Handling
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Safety
For Research Use Only (RUO). Not intended for diagnostic, therapeutic, or veterinary use. Handle according to standard laboratory biosafety guidelines. Avoid skin and eye contact. Dispose of waste in accordance with local regulations.
This antibody has been validated for: Western Blot, Immunofluorescence. For each application, follow the recommended dilutions on the product datasheet and optimize empirically for your sample type.
WB,1:500 - 1:2000|IF/ICC,1:50 - 1:100
This antibody has been confirmed reactive against: Human, Mouse, Rat. Cross-reactivity with other species may occur due to sequence homology but is not guaranteed without independent validation.
Recombinant fusion protein of human SHARPIN(NP_112236.3).
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Avoid repeated freeze-thaw cycles. Centrifuge vials briefly before opening. Dilute immediately before use.
Customization & Add-ons: Can't find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
