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Scientific Background
It has been shown that the bisphosphorylated insulin receptor substrate 1 (IRS-1) binds to the (N)-SH2 domain of SHP2 (PTPN11) resulting in activation of the SHP2 enzymatic activity.
Product Description
Recombinant ProteinRecombinant SHP-2 Activating Peptide (IRS1_pY1172(dPEG8)pY1222) protein is produced using a validated recombinant expression system and supplied in aqueous buffer solution. Suitable for enzyme kinetics, inhibitor screening, binding assays, structural studies, and related biochemical research applications.
Protein Specifications
| Purity | >95% (by HPLC) |
|---|---|
| Formulation | 100 μM peptide in DMSO |
| Storage | Soluble in DMSO. Store at -20°C. |
| Biosafety Level | Not applicable (BSL-1) |
Safety & Handling
FOR RESEARCH USE ONLY (RUO). Not for diagnostic or therapeutic applications.
This protein was produced in a recombinant expression system. Expression system selection determines post-translational processing, disulfide bond formation, and co-factor incorporation — all of which affect enzymatic activity. Insect cell (Sf9) systems are preferred for kinases and multi-subunit enzymes that require phosphorylation or chaperone assistance; E. coli is used for structurally simpler proteins.
The expressed amino acid region is specified in the product description. Confirm the region includes your domain of interest — the active site, binding pocket, or substrate recognition sequence — before placing your order. Refer to the UniProt database for domain annotation.
>95% (by HPLC) as determined by SDS-PAGE. A gel image is provided with each lot. BPS Bioscience performs rigorous QC on each lot, including purity assessment and functional activity testing where applicable. Contact technical support if purity ≥99% is required for biophysical measurements.
This protein is suitable for biochemical assays including enzyme kinetics, inhibitor screening, protein binding studies, and drug discovery. Refer to the product datasheet for validated protocols and recommended assay conditions. Contact BioHippo technical support for application-specific guidance.
Soluble in DMSO. Store at -20°C. Avoid repeated freeze-thaw cycles — prepare single-use working aliquots. Add BSA or glycerol to aliquots if storing diluted enzyme is necessary. Typical stability is at least 6 months at −80°C.
BioHippo offers flexible sourcing for qualified research institutions and partners.
- Bulk quantities: Large-scale orders for HTS campaigns or structural studies.
- Custom constructs: Alternative tag positions, truncation variants, or point mutants may be available upon request.
- Biotinylated variants: Avi-Tag site-specific biotinylation is available for SPR/BLI surface capture applications.
- Extended QC data: Activity assay data, SEC-HPLC profiles, or additional purity methods available on request.
Contact BioHippo customer support for custom requirements.
- Chen Y-N et al., Nature, 535(7610):148-152 (2016)
