| Field | Specification |
|---|---|
| Mfr No | |
| Accession Number | |
| Product Type | |
| Reporter | |
| Selection Marker | Puromycin + Blasticidin |
| Shipping | |
| Species |
Background
SIGLEC10 is a sialic acid-binding immunoglobulin-type lectin receptor expressed on macrophages and other immune cells. It carries inhibitory ITIM motifs and, upon engaging sialylated ligands such as CD24, transmits a suppressive signal that dampens macrophage activation and phagocytosis. This CD24-SIGLEC10 axis functions as a 'don't eat me' checkpoint, and tumors can exploit it to evade immune clearance. The reporter in this set uses NF-κB response elements; NF-κB is an inducible transcription factor that integrates signals from immune receptors to drive expression of inflammatory and activation genes, providing a quantitative readout of how SIGLEC10 engagement modulates downstream signaling.
Product Description & Applications
The SIGLEC10/NFκB Reporter Lentivirus Set is an immunotherapy reporter system supplied as two vials. A receptor lentivirus constitutively expresses human SIGLEC10 with antibiotic selection, while a reporter lentivirus carries tandem NF-κB response elements driving a dual reporter, secreted Gaussia luciferase together with eGFP or mCherry. Sequential transduction and selection generates a dual-stable cell line for monitoring SIGLEC10-modulated NF-κB activity.
Secreted Gaussia luciferase accumulates in conditioned media, allowing kinetic sampling without cell lysis, while fluorescence supports microscopy and flow cytometry. The VSV-G-pseudotyped particles are purified by PEG precipitation and sucrose gradient centrifugation and transduce difficult-to-transfect cells, including primary and thawed cultures. The system supports macrophage inhibition and immune-evasion assays and screening of agents that target the SIGLEC10 checkpoint.
About This Product
This 2-vial immunotherapy reporter system consists of a Vial 1 Receptor Lentivirus encoding human SIGLEC10 under a constitutive promoter with antibiotic selection, and a Vial 2 Reporter Lentivirus encoding tandem NFAT (or NF-κB) response elements driving a dual reporter (Gaussia Luciferase + eGFP, Gaussia Luciferase + mCherry). Sequential transduction and selection generates a dual-stable effector cell line that responds quantitatively to receptor stimulation with a ratiometric fluorescent + bioluminescent readout.
Secreted Gaussia luciferase (where included) accumulates in conditioned media, enabling kinetic sampling without cell lysis. The combined fluorescent and luminescent outputs allow parallel microscopy-based visualization and plate-reader luminometry from the same cell population — providing assay redundancy and flexibility for potency testing formats compliant with regulatory expectations for cell-based functional assays.
Can't find the lentiviral construct you need, or want to adjust key design elements? Contact us to discuss custom LV design and optional add-ons.
Common customization requests
- Insert / payload: replace the gene/sequence, swap to a different isoform, add mutations, or optimize cloning features.
- Expression design: change promoter (e.g., CMV/EF1α/PGK), add enhancers, or adjust regulatory elements.
- Reporters: add/swap GFP/RFP/mCherry/luciferase (single or dual reporters where applicable).
- Selection markers: add/swap puromycin/blasticidin/neomycin or fluorescent selection options.
- Vector format: switch between OE, shRNA, CRISPR (sgRNA/Cas systems), or control vectors (where supported).
Add-ons you can request
- Control viruses: empty vector, non-targeting shRNA, reporter-only controls, or matched backbone controls.
- Packaging / format: concentration options, aliquoting, or custom fill volume for screening workflows.
- Documentation: construct map/sequence confirmation package (as available) and batch documentation.
What to include in your request
- Target cell type/model (cell line or primary cells) and intended readout (reporter, knockdown, OE, etc.)
- Insert sequence (FASTA) or reference ID, plus any required tags/mutations
- Promoter, reporter, and selection marker preferences
- Desired scale and preferred format (aliquots / concentration requests)
Email us at support@biohippo.com or use the Talk to a Scientist request form.
