Sin Nombre Virus Glycoprotein (GnΔTM)

What it means: The expression system is the host platform used to produce this recombinant protein. This SNVGnΔTM antigen is expressed in mammalian cells, which shapes its structure and post-translational modifications.

Why it matters:

• Expression in mammalian cells determines post-translational modifications such as glycosylation, which is critical for preserving the native conformation of viral surface glycoproteins like SNV Gn.
• Mammalian cell-derived glycoproteins more closely mimic native virus-surface presentation compared to bacterial or insect cell expression, improving antigenicity in ELISA and neutralization assays.
• The expression system also influences endotoxin levels and downstream assay compatibility.

How to interpret here: The mammalian cell expression of this Sin Nombre Virus GnΔTM ensures high-fidelity glycoprotein folding and antigenicity appropriate for serological and assay development applications.

What it means: Form describes the physical state in which the product is supplied. This protein ships as a liquid preparation in formulation buffer (20 mM TrisHCl pH 8, 0.15 M NaCl, 5% Sucrose).

Why it matters:

• The liquid form means the product is ready to use without reconstitution — aliquot directly and use in ELISA coating or Western Blot loading.
• The sucrose-containing buffer provides cryoprotection; aliquot into single-use volumes to avoid repeated freeze-thaw cycles.
• Plan cold-chain receipt and immediate freezer storage upon delivery.

How to interpret here: Receive, aliquot, and store at -80°C immediately. Thaw on ice before each experiment. This liquid formulation of SNVGnΔTM requires no reconstitution.

What it means: The target pathogen indicates the infectious agent this antigen is derived from — Sin Nombre Virus (SNV), a New World hantavirus and the primary cause of Hantavirus Pulmonary Syndrome (HPS) in North America.

Why it matters:

• Correct Sin Nombre Virus antigen selection is fundamental to hantavirus serology, antibody characterisation, and HPS diagnostic assay development.
• SNV Gn is the major surface attachment glycoprotein and the primary target of neutralising antibodies — it is distinct from other hantavirus antigens and not cross-reactive with Old World hantaviruses in all assay formats.
• Document antigen provenance so results can be compared across studies and lots.

How to interpret here: This antigen represents the Sin Nombre Virus Gn glycoprotein (ΔTM) and is suited for SNV-specific immunological assays validated by the manufacturer for ELISA and Western Blot.

What it means: Concentration states the amount of protein supplied per unit volume. This product is provided at Batch dependent within range: 100 µg at 0.3-3 mg/mL.

Why it matters:

• Knowing the concentration lets you calculate accurate dilutions, coating amounts and assay inputs for ELISA or Western Blot.
• A batch-dependent value means the precise figure varies by lot — read the exact concentration from the lot-specific certificate of analysis or datasheet included with the product.
• Accurate concentration data underpins reproducible quantitative work and inter-lot comparison.

How to interpret here: Each 100 µg unit of this Sin Nombre Virus GnΔTM falls within the 0.3-3 mg/mL range. Confirm the exact lot concentration from the CoA before setting up assay dilutions.

What it means: Storage gives the manufacturer-recommended conditions: -20°C for up to 1 week and -80°C for long-term storage, per the IBT Bioservices datasheet.

Why it matters:

• Viral glycoproteins degrade rapidly at -20°C over extended periods; -80°C is required to maintain antigenicity and assay performance across multiple uses.
• Repeated freeze-thaw cycles are a common, avoidable cause of reduced protein activity — aliquot into single-use volumes on receipt.
• The formulation buffer contains 5% sucrose as a cryoprotectant, supporting -80°C storage stability.

How to interpret here: Upon receipt, immediately aliquot this SNVGnΔTM into single-use volumes and store at -80°C. Use -20°C only for short-term convenience up to 1 week.