| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | SIN1 / SAPK interacting protein 1 / MAPKAP1 |
| Clonality | |
| Host | |
| Immunogen | A recombinant protein fragment from the C-terminal region of human SIN1 was used as the immunogen for this antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target |
Overview
SIN1 antibody supplied as a purified reagent for WB in Human, Mouse, Rat, Dog samples. This product is a polyclonal (rabbit origin) antibody (host: Rabbit; isotype: Rabbit IgG) intended for research use only.
Key elements and design rationale
- Antibody identity: Polyclonal (rabbit origin); host Rabbit; isotype Rabbit IgG.
- Format and purification: format: Purified; purity: Protein A purified antibody.
- Species reactivity (reported): Human, Mouse, Rat, Dog.
- Applications (listed): WB.
- Immunogen / epitope context: A recombinant protein fragment from the C-terminal region of human SIN1 was used as the immunogen for this antibody..
These attributes help you align the antibody with the biological question (target state, sample type, and readout) while keeping interpretation grounded in appropriate controls.
Biological background
SIN1 is the intended antigen for this primary antibody. Reported biological context includes: mTOR, or Mammalian target of rapamycin, a large protein kinase, is a regulator of cell growth and survival via its mTORC1 (mTOR + GBL + Raptor) and mTORC2 (mTOR + GBL + SIN1 + Rictor) complexes. SIN1 is an essential component of mTORC2, which activates Akt by phosphorylating Ser473.
Research relevance and current trends
- Post-translational modification mapping: phosphorylation-site–resolved antibodies are used to connect signaling inputs to target activation states and downstream readouts.
- Signal-flow and turnover studies: researchers pair immunodetection with perturbations that modulate enzymatic activity or proteostasis to understand regulation, stability, and feedback.
- Spatial and single-cell approaches: imaging-based and cytometry workflows increasingly quantify heterogeneity and relocalization rather than only bulk abundance.
Common research applications
- Western blot (WB): compare relative abundance/isoform patterns across conditions and sample types; band shifts may reflect processing or post-translational modification.
Across these readouts, differences in signal intensity, localization, or complex enrichment are typically interpreted alongside sample-matched controls and independent evidence to distinguish regulation from technical variation.
Notes for experimental interpretation
- Isoforms, cleavage products, or post-translational modifications can alter apparent molecular weight and subcellular distribution; interpret bands and staining patterns in the context of expected biology and sample preparation.
- Species differences and epitope conservation may affect binding; use matched positive controls and orthogonal evidence when comparing across organisms.
- Control concepts: include appropriate isotype and secondary-only controls (for imaging), and consider genetic perturbations (knockout/knockdown/overexpression) or independent antibodies targeting distinct epitopes to strengthen conclusions.
Epitope context is defined by the immunogen description; when available, align this with known domains, PTM sites, or family homology to anticipate potential cross-reactivity patterns. As a polyclonal antibody, recognition spans multiple epitopes, which can improve detection across conformations but may broaden background depending on sample context.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
