| Field | Specification |
|---|---|
| Cellosaurus | CVCL_0525 |
| Species | |
| Applications | |
| Classification | |
| Tissue | |
| Disease | Adenocarcinoma |
| Age | |
| Sex | |
| Ethnicity | |
| Growth properties | |
| Biosafety level | |
| Catalog no. (Mfr.) | |
| Main SKU |
The SK-HEP-1 cell line is a cancer cell line derived from a liver adenocarcinoma in a 52-year old Caucasian man. It has been shown to form tumors in immunocompromised mice, produce fibronectin, alpha-1 protease inhibitor, and Interleukin-1. However, there is an alternative hypothesis that the cells are of endothelial origin and not hepatocytes.
—
- Isoenzymes: Me-2, 1-2, PGM3, 1, PGM1, 2, ES-D, 1, AK-1, 1, GLO-1, 1, G6PD, B
- Tumorigenic: Yes, in nude mice, forms large cell carcinoma consistent with hepatoma
- Karyotype: (P11) hyperdiploid to hypotriploid (+A3, +C, +E, +F, +G, -A, -D) with abnormalities including dicentrics, acrocentric fragments, secondary constrictions, pulverizations, and large subtelocentric and submetacentric markers
- cultureMedium: EMEM (MEM Eagle), w: 2 mM L-Glutamine, w: 2.2 g/L NaHCO3, w: EBSS (Cytion article number 820100a)
- supplements: Supplement the medium with 10% FBS and 1% NEAA
- dissociationReagent: Accutase
- subculturing: Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
- seedingDensity: 1 x 104 cells/cm2
- fluidRenewal: 2 to 3 times per week
- freezeMedium: As a cryopreservation medium, use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
10%
OFF
20%
OFF
$50
OFF
FREE
SAMPLE
