| Field | Specification |
|---|---|
| Mfr No | |
| Accession Number | |
| Product Type | |
| Promoter | |
| Reporter | |
| Selection Marker | Blasticidin, Puromycin |
| Shipping | |
| Species |
Background
SMAD2 and SMAD3 are receptor-regulated SMAD proteins that transduce signals from the transforming growth factor beta (TGF-beta) family of cytokines. When TGF-beta ligands engage their type I and type II serine/threonine kinase receptors, SMAD2 and SMAD3 are phosphorylated, partner with the common mediator SMAD4, and translocate to the nucleus to regulate transcription of target genes. This pathway controls cell proliferation, differentiation, extracellular matrix production, immune regulation, and embryonic patterning. TGF-beta/SMAD2/3 signaling is central to wound healing and tissue homeostasis, and its dysregulation contributes to fibrosis, immune disorders, and cancer, where the pathway can act as either a tumor suppressor or a promoter of progression depending on context.
Product Description & Applications
The SMAD2/3 Reporter Lentivirus is a transcription factor reporter system that provides a sensitive fluorescent or luminescent readout of TGF-beta pathway activation in transduced cells. The construct places a reporter gene (firefly luciferase, GFP, RFP, Renilla luciferase, or SEAP) under the control of tandem SMAD2/3 response elements coupled to a minimal promoter and an optimized upstream enhancer that maximizes signal-to-noise. A constitutive drug selection marker (Blasticidin or Puromycin) enables generation of stable polyclonal reporter cell lines. Stable lentiviral integration delivers consistent reporter expression suitable for fluorescence microscopy, flow cytometry, or luminometry. Supplied as high-titer particles purified by PEG precipitation and sucrose gradient centrifugation, it is well suited to studying SMAD2/3 and TGF-beta signaling in primary and difficult-to-transfect cells.
About This Product
This reporter lentivirus places a Firefly Luc, GFP, Luc, Renilla Luc, RFP, SEAP reporter gene under the control of tandem consensus response elements specific for the TGF-b signaling pathway transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Puromycin) and/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.
Stable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.
Can't find the lentiviral construct you need, or want to adjust key design elements? Contact us to discuss custom LV design and optional add-ons.
Common customization requests
- Insert / payload: replace the gene/sequence, swap to a different isoform, add mutations, or optimize cloning features.
- Expression design: change promoter (e.g., CMV/EF1α/PGK), add enhancers, or adjust regulatory elements.
- Reporters: add/swap GFP/RFP/mCherry/luciferase (single or dual reporters where applicable).
- Selection markers: add/swap puromycin/blasticidin/neomycin or fluorescent selection options.
- Vector format: switch between OE, shRNA, CRISPR (sgRNA/Cas systems), or control vectors (where supported).
Add-ons you can request
- Control viruses: empty vector, non-targeting shRNA, reporter-only controls, or matched backbone controls.
- Packaging / format: concentration options, aliquoting, or custom fill volume for screening workflows.
- Documentation: construct map/sequence confirmation package (as available) and batch documentation.
What to include in your request
- Target cell type/model (cell line or primary cells) and intended readout (reporter, knockdown, OE, etc.)
- Insert sequence (FASTA) or reference ID, plus any required tags/mutations
- Promoter, reporter, and selection marker preferences
- Desired scale and preferred format (aliquots / concentration requests)
Email us at support@biohippo.com or use the Talk to a Scientist request form.
Autocrine TGFβ2 enforces a transcriptionally hybrid cell state in Ewing sarcoma.
Wrenn ED, Harris JC, Apfelbaum AA, Valenti JR, et al.
Science Advances, 2026. DOI: 10.1126/sciadv.ady0550
Product(s) used: LTV-0013
Usage: SBE/SMAD2-3 Reporter Lentivirus (LTV-0013-1S) transduced into Ewing sarcoma cells at 5 TU/cell; GFP reporter used to quantify autocrine TGFβ2-driven SMAD2/3 signaling in a transcriptionally hybrid cancer cell state.
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