SMAD3 Antibody

SKU:BHA17100576
Suppliers
NSJ Bioreagents
NSJ Bioreagents
Details Products
Overview
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Anti-SMAD3 polyclonal antibody (Rabbit Ig) for target detection, validation, and assay development. Reactivity: Human; antigen-affinity purified reagent to support WB, IF, IHC, and ELISA.
Target SMAD3
Host Rabbit
Isotype Rabbit Ig
Reactivity Human
Application(s) WB, IF, IHC, ELISA
Options selector
Catalog no. Formulation Size
F43048-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options (Formulation: In 1X PBS, pH 7.4, with 0.09% sodium azide; Size (2) — 0.08 ml, 0.4 ml)
  • Lead time: options listed as “In Stock at Manufacturer” typically ship after a short processing time (2–3 days). Timing may vary by fulfillment source.
  • Storage: Refer to the product datasheet for storage and handling.
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: follow the product datasheet storage instructions.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Mfr No F43048
Clonality
  • Polyclonal (rabbit origin)
Host Rabbit
Immunogen A portion of amino acids 127-156 from the human protein was used as the immunogen for this SMAD3 antibody.
Isotype
  • Rabbit Ig
Product Type
  • Antibodies
  • Primary Antibodies
Purity Antigen affinity
Reactivity
  • Human
Storage Aliquot the SMAD3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Target SMAD3
UniProt # P84022
SMAD3 is a receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription. Also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. Has an inhibitory effect on wound healing probably by modulating both growth and migration of primary keratinocytes and by altering the TGF- mediated chemotaxis of monocytes. This effect on wound healing appears to be hormone-sensitive. Regulator of chondrogenesis and osteogenesis and inhibits early healing of bone fractures. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator. [UniProt]
  • Format: Antigen affinity purified
  • Predicted species reactivity: Mouse, Rat, Pig, Chicken

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.

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Experience the power of Celltrypse™, c-LEcta's innovative enzyme solution for gentle and efficient cell dissociation. Request your free sample and discover a superior alternative for your cell culture workflows.

Try Celltrypse Free – Request Your Sample Today