| Field | Specification |
|---|---|
| Alternative Names | mothers against decapentaplegic homolog 5 SMA- and MAD-related protein 5 SMAD, mothers against DPP homolog 5 MAD, mothers against decapentaplegic homolog 5 mothers against decapentaplegic, drosophila, homolog of, 5 |
| Clonality | |
| Conjugate | |
| Form | Liquid |
| Host | |
| Immunogen | Recombinant fragment, corresponding to amino acids 239aa-372aa of human SMAD5 (C-terminus). |
| Isotype | |
| Product Type | |
| Reactivity | |
| Source | This product is a monoclonal antibody produced from mouse hybridoma cells. |
| Storage | |
| Target | |
| UniProt # |
Overview
This is a monoclonal anti-SMAD5 antibody raised in Mouse, with confirmed utility in ELISA, WB, ICC, FC. It is designed to detect SMAD5 protein in Human, Pig, Rat, Mouse and supports researchers working in signal transduction contexts.
Key elements and design rationale
- Immunogen: Recombinant fragment, corresponding to amino acids 239aa-372aa of human SMAD5 (C-terminus). — determines the epitope region; confirm compatibility with sample preparation and expected post-translational modifications.
- Host species (Mouse): Requires anti-mouse-IgG secondary reagents for indirect detection.
- Monoclonal format: Single-epitope binding provides high specificity and lot-to-lot reproducibility. Confirm epitope accessibility under your assay conditions.
- Isotype (IgG1): Matched secondary antibodies and isotype controls required. Compatible with standard Protein A/G purification workflows.
- Purification (Antigen affinity purification): Enriches for specific immunoglobulin classes; reduces non-specific background vs. crude antisera.
Biological background
SMAD5 (also referred to as mothers against decapentaplegic homolog 5 SMA- and MAD-related protein 5 SMAD, mothers against DPP homolog 5 MAD, mothers against decapentaplegic homolog 5 mothers against decapentaplegic, drosophila, homolog of, 5) is a protein target studied in Human systems. Expression, subcellular localization, and post-translational modifications vary across cell types and disease states — factors critical to antibody-based detection design. Consult UniProt, NCBI Gene, and primary literature for current annotation of SMAD5 biology in signal transduction.
Common research applications
- ELISA: Quantification of soluble target in biological fluids or culture supernatants. Ensure samples are within the linear detection range.
- WB: Confirms target molecular weight and distinguishes isoforms under denaturing conditions.
- ICC: Subcellular localization in fixed cells or tissue sections; fixation and permeabilization conditions affect epitope accessibility.
- FC: Applicable for FC-based detection; refer to product documentation for optimized conditions.
Notes for experimental interpretation
- Isotype controls: Use an isotype-matched (IgG1 from Mouse) control at equivalent concentration to assess non-specific background.
- Cross-reactivity: Confirm the clone does not cross-react with related family members under your assay conditions.
- Matrix effects: Sample matrix can affect performance; pilot dilution linearity and spike-recovery experiments are advised for quantitative studies.
- Species reactivity: Confirmed for Human, Pig, Rat, Mouse. Extrapolation to untested species requires empirical validation.
This product is a monoclonal antibody produced from mouse hybridoma cells.
SMAD5 is a protein target in Human, Pig, Rat, Mouse biology. This monoclonal antibody raised in Mouse is designed to detect SMAD5 in ELISA, WB, ICC, FC applications, with IgG1 isotype.
Reported reactive against Human, Pig, Rat, Mouse. Immunogen derived from Human. Cross-reactivity with other species should not be assumed without documented data or empirical testing.
Reported for ELISA, WB, ICC, FC. Each format requires independent dilution optimization and appropriate controls. Unlisted applications require empirical testing.
This non-conjugated IgG1 antibody requires a compatible secondary. Select anti-mouse IgG secondary conjugated to your preferred reporter (HRP, AP, fluorophore, or biotin), matched to IgG1.
(1) Isotype control — IgG1 from Mouse at matching concentration; (2) Positive control — known SMAD5-expressing cell/tissue; (3) Negative control — knockdown/knockout sample for specificity; (4) Dilution linearity — verify proportional signal decrease in your matrix.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
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