| Field | Specification |
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| Clonality | |
| Host | |
| Immunogen | E.coli-derived human SMARCAL1 recombinant protein (Position: M1-L782) was used as the immunogen for the SMARCAL1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
SMARCAL1 Antibody / HARP / HepA-related protein is a anti-SMARCAL1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Nuclear.
Key elements and design rationale
- Target: SMARCAL1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, FACS, ELISA
Biological background
SMARCAL1 is a member of the SNF2 family of chromatin remodelers, a large group of ATPases that modify DNA-protein interactions to facilitate replication and transcription. It is recruited to sites of replication fork arrest via interactions with replication protein A (RPA) and catalyzes branch migration to restart replication. This enzymatic process helps prevent double-strand breaks and genomic collapse. The SMARCAL1 antibody allows direct visualization of the protein in the nucleus, providing insight into its dynamics during S-phase and after genotoxic stress.
Mutations in SMARCAL1 are the primary cause of Schimke immuno-osseous dysplasia (SIOD), a rare autosomal recessive disorder marked by short stature, renal failure, and immune dysfunction. These phenotypes stem from impaired DNA repair and replication mechanisms, demonstrating the importance of SMARCAL1 in cellular viability and developmental stability. The SMARCAL1 antibody is therefore valuable not only in basic chromatin biology but also in studies of human disease and genomic maintenance.
SMARCAL1's helicase activity is regulated by phosphorylation events mediated by ATR kinase and other components of the DNA damage response. These modifications influence its recruitment and activity at stalled forks. Researchers use the SMARCAL1 antibody in combination with phospho-specific markers to map these regulatory pathways. Western blotting enables detection of total protein levels, while immunofluorescence and confocal microscopy reveal its nuclear localization and accumulation at replication foci. Chromatin immunoprecipitation using the SMARCAL1 antibody further allows identification of genomic sites directly bound by the enzyme, shedding light on its mechanistic involvement in replication restart.
In broader biological contexts, SMARCAL1 contributes to genome stability, epigenetic maintenance, and transcriptional regulation through chromatin remodeling. Its deficiency can cause widespread transcriptional dysregulation and heightened sensitivity to replication stress. The SMARCAL1 antibody supplied by
Ongoing research using the SMARCAL1 antibody continues to clarify how this enzyme coordinates DNA replication and repair, how its dysfunction leads to disease, and how it might be therapeutically targeted to enhance genome stability. The combination of functional versatility and high-quality specificity makes the SMARCAL1 antibody a key reagent for understanding the complex interplay between chromatin structure, replication stress, and genomic maintenance.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
