| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | SMPD4;NET13;NSMASE-3;NSMASE3 |
| Conjugate | |
| Formulation | |
| Host | |
| Immunogen | Recombinant fusion protein of human SMPD4(NP_060421.2). |
| Molecular Weight | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage |
Product Overview
Research Use OnlyThe protein encoded by this gene is a sphingomyelinase that catalyzes the hydrolysis of membrane sphingomyelin to form phosphorylcholine and ceramide. This gene is activated by DNA damage, cellular stress, and tumor necrosis factor, but it is downregulated by wild-type p53. The encoded protein localizes to the endoplasmic reticulum and Golgi network.
Validated Applications
| Western Blot (WB) | 200 - 1:2000|IF/ICC |
|---|---|
| Immunofluorescence (IF/ICC) | 50 - 1:200 |
Key Specifications
| Host Species | Rabbit |
|---|---|
| Species Reactivity | Human, Mouse |
| Conjugate | Unconjugated |
| Purification | Affinity Purification |
| Formulation | 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 |
| Molecular Weight | ~ 100 kDa |
| Catalog No. | BS72607 |
| UniProt | Q9NXE4 |
| Alternative Names | SMPD4;NET13;NSMASE-3;NSMASE3 |
Immunogen / Antigen
Recombinant fusion protein of human SMPD4(NP_060421.2).
Reactivity Notes
Polyclonal Antibodies
Storage & Handling
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Safety
For Research Use Only (RUO). Not intended for diagnostic, therapeutic, or veterinary use. Handle according to standard laboratory biosafety guidelines. Avoid skin and eye contact. Dispose of waste in accordance with local regulations.
This antibody has been validated for: Western Blot, Immunofluorescence. For each application, follow the recommended dilutions on the product datasheet and optimize empirically for your sample type.
WB,1:200 - 1:2000|IF/ICC,1:50 - 1:200
This antibody has been confirmed reactive against: Human, Mouse. Cross-reactivity with other species may occur due to sequence homology but is not guaranteed without independent validation.
Recombinant fusion protein of human SMPD4(NP_060421.2).
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Avoid repeated freeze-thaw cycles. Centrifuge vials briefly before opening. Dilute immediately before use.
Customization & Add-ons: Can't find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
