| Field | Specification |
|---|---|
| Mfr No | |
| Product Type | |
| Shipping | |
| Source | Recombinant (E. coli) |
| Storage |
Snailase is a mixture of enzymes containing more than 20 enzymes including cellulase, pectinase, amylase, and protease. It can be used for yeast cell wall disruption, and is therefore widely applied in cell biology and genetic engineering research. It can also be used as a feed additive to improve feed digestibility, or in juice clarification, debittering of citrus fruits, and jam production.
After treatment with a sulfhydryl compound, yeast cells are suspended in an isotonic sorbitol solution at pH 5.8–7.2. Under these conditions, 30–40 mg of enzyme per gram of cells at 37°C for 1 hour will dissolve the cell wall with a disruption efficiency exceeding 90%.
Features
Multi-Enzyme Composition: Contains more than 20 enzymes, including cellulase, pectinase, amylase, and protease, for effective yeast cell wall degradation.
High Efficiency: 30–40 mg enzyme per gram of cells at 37 °C for 1 hour achieves over 90% cell wall digestion.
Reliable Performance: Delivers consistent cell lysis results, enabling downstream applications such as nucleic acid extraction and protoplast preparation.
Components
|
Name |
10404ES08 |
10404ES10 |
|
Snailase |
5 g |
10 g |
Storage
This Product should be stored at 2~8℃ for 2 years.
Application
yeast cell wall digestion; nucleic acid extraction; and fungal biotechnology applications.
Glycerol Content: Contains Glycerol
Store this enzyme at -20°C and avoid repeated freeze-thaw cycles to preserve catalytic activity. The product is shipped Dry Ice and remains stable for up to one year from the date of manufacture when stored under recommended conditions. Aliquoting the stock solution into single-use volumes is recommended for enzymes used infrequently to minimize thermal cycling of the bulk stock.
This enzyme is validated for use in NGS Library Preparation applications. It is suitable for use in both standard laboratory research settings and, where applicable, optimized reaction workflows requiring high specificity, sensitivity, or throughput.
Activity is expressed in lysate units (LU) or U/mg, defined as the amount of enzyme that produces a defined reduction in optical density (OD₆₀₀) of a bacterial suspension per minute under standard assay conditions.
This enzyme is produced as Recombinant (E. coli) and supplied as a Research Use Only (RUO) reagent. Each lot is subjected to activity assay, purity assessment by SDS-PAGE, and functional validation prior to release. A Certificate of Analysis (CoA) and Safety Data Sheet (SDS) are available on request.
Lytic enzymes such as lysozyme cleave the β-1,4-glycosidic linkage between N-acetylmuramic acid and N-acetylglucosamine in bacterial peptidoglycan cell walls, releasing intracellular contents for downstream nucleic acid or protein extraction. Mutanolysin targets β-1,4-glycosidic bonds in the muramidase substrate of Gram-positive bacteria resistant to lysozyme. Enzyme lysis is typically gentler than mechanical disruption and reduces shear-induced DNA fragmentation.
Yeasen Biotechnology supports custom enzyme solutions across multiple service lines — from GMP-grade bulk supply to directed enzyme engineering. Contact BioHippo to discuss requirements and initiate a project inquiry.
▶ GMP-Grade & Bulk Supply
Select Yeasen enzymes are available in GMP grade, manufactured in an ISO 13485-certified UCF.ME™ ultra-clean molecular enzyme facility with FDA Drug Master File (DMF) support.
- GMP-grade release testing and CoA documentation
- ISO 13485-certified production facility
- Scalable from milligram to multi-gram quantities
- Consistent lot-to-lot activity specifications
▶ Glycerol-Free & Custom Formulation
Glycerol-free enzyme formats are available for applications requiring lyophilization compatibility, liquid handling automation, or direct IVD master mix integration.
- Glycerol-free liquid format (standard and custom buffers)
- Lyophilization-ready enzyme preparation
- Custom reaction buffer optimization for specific assay conditions
- Compatible with freeze-drying workflows for point-of-care formats
▶ Molecular IVD RDC Service
Yeasen's Research and Development Contracting (RDC) team delivers end-to-end solutions for molecular diagnostic product development, covering enzyme selection through clinical validation support.
- Enzyme selection and performance matching
- Primer/probe design and reaction buffer optimization
- Sensitivity, specificity, and precision validation studies
- Stability studies and SNP evaluation
- Instrument platform compatibility assessment
▶ ZymeEditor™ Enzyme Engineering
Yeasen's proprietary ZymeEditor™ directed evolution and rational design platform enables the development of custom enzyme variants with tailored performance characteristics not available in off-the-shelf products.
- Directed evolution for enhanced thermostability, processivity, or fidelity
- Rational design for altered substrate specificity or cofactor requirements
- Library screening from Yeasen's proprietary enzyme variant collection
- Scale-up to commercial quantities upon candidate confirmation
ⓘ Customization services are fulfilled by Yeasen Biotechnology. Lead times and minimum order quantities vary by service type. Contact BioHippo for project scoping and pricing.
