SNU-16 cell

The SNU-16 cell line is derived from a poorly differentiated gastric carcinoma of a human adult. This cell line is extensively used in gastric cancer research, offering a model to study the molecular and cellular mechanisms involved in the development and progression of gastric adenocarcinoma. SNU-16 cells are particularly valuable for investigating genetic alterations, signal transduction pathways, and the tumor microenvironment associated with this aggressive form of stomach cancer. SNU-16 cells exhibit an epithelial morphology and are characterized by the expression of gastric carcinoma markers, including carcinoembryonic antigen (CEA) and various cytokeratins. They are known to possess amplification of the c-MET gene and overexpression of the MET receptor, which plays a significant role in cell growth, survival, and metastasis. Researchers use SNU-16 cells to explore the role of the MET signaling pathway in gastric cancer and to evaluate the efficacy of MET inhibitors and other targeted therapies. Additionally, SNU-16 cells are utilized in drug resistance studies, high-throughput screening assays, and preclinical testing of new chemotherapeutic agents. The relevance of the SNU-16 cell line in gastric cancer research underscores its importance in advancing our understanding of the disease and developing more effective treatment strategies for gastric cancer patients.

SKU:BHC11101451

• SDS (019416EC35687F248882D4594AF919C0)
• Product Sheet (019416EC35687F248882D4594AF919C0)
• SNU-16 305273-021025 Cytion CoA (PDF)

• Surface antigens: Blood Type A, Rh +, carcinoembryonic antigen (CEA) and TAG 72
• Oncogenes: myc +, erb-B2 +
• Tumorigenic: Yes, in semisolid medium
• Mutational profile: Mutation: MSH6, p.Lys1358fs*2 (c.4065_4066insTTGA), heterozygous; Mutation: TP53, p.Tyr205Phe (c.614A>T), homozygous

• cultureMedium: RPMI 1640, w: 2.0 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a)
• supplements: Supplement the medium with 10% FBS, 25 mM HEPES
• subculturing: Suspension cells: Remove cells from substrate by pipetting with fresh medium. To obtain single cells, pass the suspension several times through a 22 gauge needle and dispense into new flasks.
• fluidRenewal: 2 times per week
• freezeMedium: As a cryopreservation medium, use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.