| Field | Specification |
|---|---|
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Product Overview
This product is suitable for the isolation of total DNA from 180–220 mg of fresh or frozen soil. Once the sample has been lysed, the genomic DNA of the organisms present in the soil, including animal, plant, viral, bacterial and parasite DNA, binds to the nucleic acid purification column. Degraded proteins and PCR inhibitors are filtered out. After washing with Wash Buffer and Solution RP, the genomic DNA is eluted with Elution Buffer and can be used in downstream molecular biology experiments.
The protocol takes 180–220 mg of soil through lysis at 95°C for 5 min, clarification and column binding, washing and elution in 50–200 µL, with the elution centrifuged at 12,000 rpm.
Specifications
| Format | Spin column (G column) |
|---|---|
| Sample type | Fresh or frozen soil |
| Sample input | 180–220 mg |
| Nucleic acids recovered | Genomic DNA of soil organisms: animal, plant, viral, bacterial and parasite DNA |
| Elution volume | 50–200 µL |
Kit Components
| Component | 50 T | 200 T |
|---|---|---|
| Solution SGE | 80 mL | 160 mL × 2 |
| Solution InR | 15 mL | 60 mL |
| Solution GA2 | 12 mL | 48 mL |
| Wash Buffer | 70 mL | 280 mL |
| Solution RP | 25 mL | 100 mL |
| Elution Buffer | 10 mL | 40 mL |
| Proteinase K | 525 µL | 2.1 mL |
| G column | 50 set | 200 set |
Applications
- Total DNA extraction from fresh or frozen soil
- Recovery of bacterial and parasite DNA from soil
- Recovery of viral DNA from soil
- Downstream molecular biology experiments on soil DNA
Storage & Handling
All components are stored at room temperature for 1 year, except Proteinase K, which is stored at -20°C for 1 year.
Usage Notes
- Store freshly collected soil samples at -20°C or lower without delay.
- Storage at room temperature causes DNA degradation within hours.
- Use 180–220 mg of soil per preparation.
- Carry out the lysis step at 95°C for 5 min as specified.
Safety & Regulatory
For research use only. Not intended for clinical, diagnostic or therapeutic use. Follow the manufacturer's manual and standard laboratory safety practice.
Customization & Add-ons: Need this molecular biology reagent tailored to your workflow? We can help with bulk or custom pack sizes, alternative formats (spin column, magnetic bead or reagent-only kits; premixed or stand-alone reference dye), matched extraction, cDNA synthesis and qPCR workflows, and sourcing of related enzymes, ladders and consumables not in our catalog. Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services — our team will follow up with feasibility details and next steps.
