| Field | Specification |
|---|---|
| Target | |
| Alternative names | Hyaluronidase PH-20 (Hyal-PH20)|Hyaluronoglucosaminidase PH-20|Sperm adhesion molecule 1|Sperm surface protein PH-20|SPAM1|HYAL3|PH20 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | sperm adhesion molecule 1(PH-20 hyaluronidase, zona pellucida binding) |
| Conjugate | |
| Molecular weight | |
| Purity | |
| Purification | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Scientific Background
Involved in sperm-egg adhesion. Upon fertilization sperm must first penetrate a layer of cumulus cells that surrounds the egg before reaching the zona pellucida. The cumulus cells are embedded in a matrix containing hyaluronic acid which is formed prior to ovulation. This protein aids in penetrating the layer of cumulus cells by digesting hyaluronic acid.
Product Overview
This polyclonal antibody, raised in Rabbit, recognizes SPAM1. It is validated for ELISA, Western Blot.
Validated Applications
| Applications | ELISA, Western Blot |
|---|---|
| WB | 1:500-1:2000 |
Optimal dilutions should be determined by the end user for each application and sample type.
Immunogen
sperm adhesion molecule 1(PH-20 hyaluronidase, zona pellucida binding)
Specifications
| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Immunogen | sperm adhesion molecule 1(PH-20 hyaluronidase, zona pellucida binding) |
| Purification | Immunogen Affinity Purified |
| Purity | ≥95% as determined by SDS-PAGE |
| Form | Liquid |
| Target MW | 50 kDa |
| UniProt # | P38567 |
| Gene ID | 6677 |
| Storage | PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.) |
| Alternative Names | Hyaluronidase PH-20 (Hyal-PH20)|Hyaluronoglucosaminidase PH-20|Sperm adhesion molecule 1|Sperm surface protein PH-20|SPAM1|HYAL3|PH20 |
Reactivity Notes
Validated reactivity: Human, Mouse, Rat. For other species, epitope conservation may allow cross-reactivity; confirm with appropriate controls.
Safety & Handling
This product is for research use only (RUO) and is not intended for diagnostic or therapeutic use. The formulation contains sodium azide; handle with standard laboratory precautions.
Related Products
Explore related antibodies and reagents for SPAM1 research from Fine Test, or contact us for alternative formats.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
