Spin-Column Membrane Protein Folding Screen Kit

What it means: 20 folding conditions — how many different conditions the product lets you test.

Why it matters:

• Different proteins need different conditions, so a screen improves the chance of finding one that works.
• Testing several conditions side by side saves time compared with testing them one by one.
• The best condition from a screen can then be scaled up for preparative work.
• Running all conditions with the same sample makes the results directly comparable.
• Recording the condition number lets you reorder exactly the condition that worked.
• Screening also shows which conditions do not work, which narrows later optimization.
• A broader screen is most useful when little is known about how a protein behaves.

For this product: The kit screens 20 folding conditions (columns #1–20) with detergents and lipids that form micelles or bicelles.

Tip: Record the column number of the best condition; it is the number you order for scale-up.

What it means: Prepacked spin columns — the physical format of the product.

Why it matters:

• The format decides which equipment and steps you need.
• It tells you how many samples or conditions you can process at once.
• Knowing the format helps you plan bench time and handling steps.
• It helps you compare this product with others used for the same task.
• A familiar format shortens setup time for new users.
• The format also decides how much hands-on time each run takes.
• Choosing the right format avoids repeating work at a larger scale.

For this product: Prepacked spin columns, one per condition, are used in a bench-top microcentrifuge.

Tip: Incubate the eluates at 4°C, as described in the protocol.

What it means: 25 µl per column — the sample volume the product handles.

Why it matters:

• The volume decides how much starting material you need.
• Staying within the stated volume keeps performance as described in the protocol.
• It helps you plan dilution or concentration steps before you start.
• Using the same volume in every run makes results easy to compare.
• Larger samples can be split across several units or runs.
• Recording the volume used makes yields and recoveries easy to compare.
• Measuring the volume carefully keeps ratios to reagents as intended.

For this product: Load 25 µl per column of loading sample (solubilized inclusion bodies mixed 1:1 with Reagent A or B).

Tip: Use Reagent A for columns #1–10 and Reagent B for columns #11–20.

What it means: Frozen — how the product is shipped.

Why it matters:

• The shipping condition tells you what to expect when the package arrives.
• Unpacking promptly lets you check the contents against the order.
• Checking the package on arrival helps you spot transport problems early.
• Storage conditions can differ from shipping conditions, so confirm them before the package arrives.
• Recording the arrival date and condition helps if questions come up later.
• Arranging for someone to receive the package avoids delays when it arrives.
• The shipping condition is chosen to keep the product as described during transport.

For this product: Ships frozen; unpack it promptly on arrival. Please contact us to confirm storage conditions.

Tip: Open and check the package on arrival, and contact us right away if anything is damaged or missing.