Spin-Columns for Liposome Purification

What it means: Spin Column — the column format used to process each sample.

Why it matters:

• Spin columns need only a bench-top microcentrifuge and 1.5 ml tubes as receivers.
• Each column processes one sample, so several samples can be run in parallel in the same rotor.
• Separation takes a few short spins instead of a gravity column run.
• Pre-packed columns avoid packing resin by hand.
• The purified liposomes are collected directly in the receiver tube.

For this product: Each spin column is pre-packed and ready to use after the preparation spins described in the ProFoldin protocol.

Tip: Cut the caps off the receiver tubes before use, as the protocol describes, so the column sits correctly during spins.

What it means: 20 columns — how many columns come in one pack.

Why it matters:

• Each column processes one 100 µl sample load, so one pack covers up to 20 samples.
• Larger sample volumes use more columns.
• Including control samples, such as a buffer-only load, uses columns from the same pack.
• Columns are used one at a time, so unused columns can stay in the pack until needed.
• Running a whole sample series from one pack keeps the column preparation the same for every sample.

For this product: Each pack of this product contains 20 columns, each pre-packed and ready for the preparation spins.

Tip: Count samples, repeats and controls before starting so that one pack covers the whole experiment.

What it means: 100 µl — the sample volume loaded onto one column per spin.

Why it matters:

• Load 100 µl of liposome sample per column after equilibrating the column with the liposome buffer.
• Each column is used for one sample and then discarded.
• Staying within the stated volume keeps separation performance as described in the protocol.
• Loading the same volume on every column makes recovery easy to compare between samples.
• Have samples ready before starting the column preparation spins.

For this product: Load 100 µl per column. Spin speeds and times are given in the ProFoldin protocol under Documents.

Tip: Measure 100 µl carefully for each load, and use the same volume for every column in a comparison.

What it means: More than 99% — how much free, non-encapsulated drug the column removes from a liposome sample.

Why it matters:

• Free drug in a liposome sample can bias encapsulation and release measurements.
• Removal combines adsorption and size exclusion, so liposomes pass into the eluate while free drug stays on the column.
• A small amount of liposomes may also bind to the column.
• The whole separation takes a few minutes in a bench-top microcentrifuge.

For this product: The column removes more than 99% of non-encapsulated drug in a few minutes. In ProFoldin testing, 99.3% of a 2 mg/ml liposomal doxorubicin sample was recovered, compared with 0.2% of free doxorubicin.

Tip: Equilibrate the column with the same buffer as the liposome preparation before loading the sample.

What it means: Cold, Not Frozen — how the product is shipped.

Why it matters:

• The shipping condition tells you what to expect when the package arrives.
• Unpacking promptly lets you check the contents against the order.
• Checking the package on arrival helps you spot transport problems early.
• Keeping the shipping box until the contents are checked makes any claim easier.
• Storage conditions can differ from shipping conditions, so confirm them before the package arrives.
• Recording the arrival date and condition helps if questions come up later.

For this product: Cold, Not Frozen: Shipped cold (not frozen). Please contact us for storage conditions.

Tip: Open and check the package on arrival, and contact us right away if anything is damaged or missing.