| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Serine palmitoyltransferase 1; Long chain base biosynthesis protein 1; LCB 1; Serine-palmitoyl-CoA transferase 1; SPT 1; SPT1; SPTLC1; LCB1 |
| Conjugate | |
| Formulation | |
| Host | |
| Immunogen | Recombinant full length Human SPTLC1. |
| Molecular Weight | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage |
Product Overview
Research Use OnlySPTLC1 (serine palmitoyltransferase 1), also known as LCB1, and SPTLC2 (serine palmitoyltransferase 2), also known as LCB2, together catalyze sphingolipid biosynthesis by converting L-serine and palmitoyl-CoA to 3-oxosphinganine, utilizing pyridoxal 5'-phosphate as a cofactor. Increases in transepidermal water loss triggers upregulation of serine palmitoyltransferase mRNA expression in humans. Deficiencies in wild type SPTLC1 and SPTLC2 can lead to hereditary sensory neuropathy, atopic eczema, and psoriasis.
Validated Applications
| Western Blot (WB) | 1:500~1:2000 |
|---|---|
| Immunohistochemistry (IHC) | 1:50~1:200 |
Key Specifications
| Host Species | Rabbit |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Conjugate | Unconjugated |
| Purification | Affinity Purification |
| Formulation | Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 |
| Molecular Weight | ~ 53 kDa |
| Catalog No. | BS8409 |
| UniProt | O15269 |
| Alternative Names | Serine palmitoyltransferase 1; Long chain base biosynthesis protein 1; LCB 1; Serine-palmitoyl-CoA transferase 1; SPT 1; SPT1; SPTLC1; LCB1 |
Immunogen / Antigen
Recombinant full length Human SPTLC1.
Reactivity Notes
SPTLC1 polyclonal antibody detects endogenous levels of SPTLC1 protein.
Storage & Handling
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Safety
For Research Use Only (RUO). Not intended for diagnostic, therapeutic, or veterinary use. Handle according to standard laboratory biosafety guidelines. Avoid skin and eye contact. Dispose of waste in accordance with local regulations.
This antibody has been validated for: Western Blot, Immunohistochemistry. For each application, follow the recommended dilutions on the product datasheet and optimize empirically for your sample type.
WB: 1:500~1:2000 IHC: 1:50~1:200
This antibody has been confirmed reactive against: Human, Mouse, Rat. Cross-reactivity with other species may occur due to sequence homology but is not guaranteed without independent validation.
Recombinant full length Human SPTLC1.
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Avoid repeated freeze-thaw cycles. Centrifuge vials briefly before opening. Dilute immediately before use.
Customization & Add-ons: Can't find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
