| Field | Specification |
|---|---|
| Mfr No | |
| Activity | |
| Cas No. | |
| Concentration | |
| Form | Lyophilized Powder |
| Product Type | |
| Purity | |
| Reconstitution | |
| Shipping | |
| Solubility | 50 mM in water. Centrifuge all product preparations before use (10000 x g 5 min). |
| Source | Synthetic |
| Storage | |
| Target |
Product details
- Chemical name: 1-(6-chloropyridin-2-yl)piperidin-4-amine hydrochloride.
- Also known as: SR 57227 hydrochloride
- CAS number: 77145-61-0
- Molecular formula: C10H15Cl2N3.
- Purity: >96%
- Concentration: 100 nM - 100 µM.
- Form: Lyophilized Powder
- Solubility: 50 mM in water. Centrifuge all product preparations before use (10000 x g 5 min).
- Reconstitution: Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity
- Shipping: Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
- Target: Serotonin-gated channels
- Source: Synthetic
- Activity: SR 57227A is a high affinity, selective 5-HT3 receptor agonist, active in vivo and CNS penetrant1.
- Alternative names: SR 57227 hydrochloride
Scientific background
SR 57227A is a compound with high affinity and selectivity for activating 5-HT3 receptors. It acts as an agonist for peripheral and central 5-HT3 receptors, both in vitro and in vivo, with IC50 values between 2.8 and 250 nM with no affinity for other subtypes of 5-HT.SD 57227A has the ability to cross the blood brain barrier, unlike many 5-HT3 receptors. Thus, SR 57227A can be used as a useful tool for characterizing the neuropharmacological effects produced by the stimulation of 5-HT3 receptors1.Some findings show that administration of SR 57227A can acutely induce and trigger movements and motor response in the hind limbs of adult paraplegic mice2.Additional findings indicate that SR 57227A exhibits antidepressant-like activity in rodent and decrease isolation induced aggressive behavior3.
Lead time: 1-2 Business Days
Country of origin: Israel/IL
Applications key
Application key: FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay.
Bioassay tested: Yes
Need this compound in a format that drops straight into your assay? We can tailor formulation, chemistry, and documentation so your results stay consistent across runs and re-orders.
- Format options: solid or pre-dissolved solution (choose solvent), target concentration, aliquots, light/moisture-protected packaging
- Chemistry options: free base/acid vs salt forms, hydrate/solvate preference, stereoisomer control (single enantiomer or racemate), close analogs
- Add-on labels & handles: D/¹³C/¹⁵N isotopes (LC-MS/internal standards), azide/alkyne or other functional handles for conjugation
- QC & documentation: standard COA or enhanced analytical pack (HPLC/LC-MS/NMR), chiral purity, residual solvents, water content (KF), method-specific specs
- Scale & continuity: mg to gram scale, bulk pricing, lot reservation, repeat-order continuity
To quote quickly, tell us: compound name + CAS/structure (SMILES or mol file), intended assay context, solvent preference, salt/stereochemistry requirements, purity/QC level, and the amount (mg–g).
Can’t find the compound you’re looking for?
Send the CAS or structure and your specs. We can help source it, suggest close equivalents, or discuss custom synthesis with the right QC documentation (RUO).
