| Field | Specification |
|---|---|
| Mfr No | |
| Accession Number | |
| Product Type | |
| Promoter | |
| Reporter | |
| Selection Marker | Blasticidin, N/A, Puromycin |
| Shipping | |
| Species |
Background
SREBP1, encoded by SREBF1, is a basic helix-loop-helix leucine zipper transcription factor that acts as a master regulator of lipid biosynthesis. Synthesized as an inactive precursor anchored in the endoplasmic reticulum membrane, SREBP1 is transported to the Golgi and proteolytically processed to release a mature, nuclear-active fragment that binds sterol regulatory elements to activate genes for fatty acid and triglyceride synthesis. A constitutively active form lacking the membrane-anchoring regions remains transcriptionally active without sterol regulation. SREBP1 signaling is central to lipid homeostasis, and its dysregulation is implicated in metabolic disorders, fatty liver disease, and cancer cell lipid metabolism.
Product Description & Applications
The SREBP1 (const. active) ORF cDNA Lentivirus delivers lentiviral particles packaged with an expression vector for the human SREBP-1a isoform in a constitutively active form. A CMV promoter drives the ORF cDNA fused to a C-terminal V5 tag, with a downstream selection marker separated by a self-cleaving peptide for independent translation from the same transcript; fluorescent (GFP, RFP) or luminescent reporter options are also available, and some constructs use a PGK promoter for the reporter and marker. ORF cloning accuracy has been confirmed by sequencing and protein expression validated by transient transfection. The vectors provide high, stable expression of active SREBP-1 and reliable genome integration for long-term stable cell lines, and efficiently transduce difficult-to-transfect cells, including primary and thawed cells, for studies of lipid metabolism.
About This Product
This ORF cDNA lentivirus enables stable overexpression of SREBF1 (NCBI Accession: NM_001321096.3, NM_004176.4) in mammalian cells via a third-generation, VSV-G pseudotyped delivery system. The ORF cDNA is fused to a C-terminal epitope tag (V5, Myc, or HA) and expressed under a strong constitutive promoter (CMV). Reporter and selection marker components (GFP, RFP; Blasticidin, Puromycin) are co-expressed via self-cleaving P2A peptides, enabling independent protein production without fusion-tag artifacts.
Ultra-purification by PEG precipitation and sucrose gradient centrifugation yields high-titer particles suitable for primary cells, suspension cultures, and stem cells. Stable polyclonal cell lines are established within 10–14 days by antibiotic selection or FACS sorting. For in vivo applications, the serum-free formulation and VSV-G envelope support direct administration or further concentration for stereotactic injection.
Can't find the lentiviral construct you need, or want to adjust key design elements? Contact us to discuss custom LV design and optional add-ons.
Common customization requests
- Insert / payload: replace the gene/sequence, swap to a different isoform, add mutations, or optimize cloning features.
- Expression design: change promoter (e.g., CMV/EF1α/PGK), add enhancers, or adjust regulatory elements.
- Reporters: add/swap GFP/RFP/mCherry/luciferase (single or dual reporters where applicable).
- Selection markers: add/swap puromycin/blasticidin/neomycin or fluorescent selection options.
- Vector format: switch between OE, shRNA, CRISPR (sgRNA/Cas systems), or control vectors (where supported).
Add-ons you can request
- Control viruses: empty vector, non-targeting shRNA, reporter-only controls, or matched backbone controls.
- Packaging / format: concentration options, aliquoting, or custom fill volume for screening workflows.
- Documentation: construct map/sequence confirmation package (as available) and batch documentation.
What to include in your request
- Target cell type/model (cell line or primary cells) and intended readout (reporter, knockdown, OE, etc.)
- Insert sequence (FASTA) or reference ID, plus any required tags/mutations
- Promoter, reporter, and selection marker preferences
- Desired scale and preferred format (aliquots / concentration requests)
Email us at support@biohippo.com or use the Talk to a Scientist request form.
