| Field | Specification |
|---|---|
| Mfr No | |
| Accession Number | |
| Product Type | |
| Promoter | |
| Reporter | |
| Selection Marker | Blasticidin, Puromycin |
| Shipping | |
| Species |
Background
SREBP1 (sterol regulatory element-binding protein 1, encoded by SREBF1) is a basic helix-loop-helix leucine zipper transcription factor and a master regulator of lipid biosynthesis. Synthesized as an inactive endoplasmic reticulum-bound precursor, SREBP1 is transported to the Golgi and proteolytically processed to release its transcriptionally active fragment in response to low cellular lipid levels and insulin signaling. The mature factor enters the nucleus and induces genes for fatty acid synthesis and lipogenesis. SREBP1 is central to hepatic and adipose lipid metabolism, and its dysregulation contributes to fatty liver disease, insulin resistance, obesity, and altered lipid metabolism in cancer.
Product Description & Applications
The SREBP1 Reporter Lentivirus places a reporter gene under the control of response elements preferentially activated by SREBP1, providing a sensitive readout of SREBP1 transcriptional activity and lipogenesis pathway activation in human and mouse cells. The reporter design may help discriminate SREBP1 from SREBP2 activity. Reporter options include fluorescent (GFP, RFP) and luminescent (firefly and Renilla luciferase) formats, with blasticidin or puromycin selection for stable reporter cell line establishment.
The particles are purified by PEG precipitation and sucrose gradient centrifugation and efficiently transduce difficult-to-transfect cells, including primary and cryopreserved cultures. Applications include monitoring lipogenic signaling, studying lipid metabolism, and screening modulators of SREBP1 activity.
About This Product
This reporter lentivirus places a Firefly Luc, GFP, Luc, Renilla Luc, RFP reporter gene under the control of tandem consensus response elements specific for the Lipogenesis pathway (SREBP1) transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Puromycin) and/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.
Stable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.
Can't find the lentiviral construct you need, or want to adjust key design elements? Contact us to discuss custom LV design and optional add-ons.
Common customization requests
- Insert / payload: replace the gene/sequence, swap to a different isoform, add mutations, or optimize cloning features.
- Expression design: change promoter (e.g., CMV/EF1α/PGK), add enhancers, or adjust regulatory elements.
- Reporters: add/swap GFP/RFP/mCherry/luciferase (single or dual reporters where applicable).
- Selection markers: add/swap puromycin/blasticidin/neomycin or fluorescent selection options.
- Vector format: switch between OE, shRNA, CRISPR (sgRNA/Cas systems), or control vectors (where supported).
Add-ons you can request
- Control viruses: empty vector, non-targeting shRNA, reporter-only controls, or matched backbone controls.
- Packaging / format: concentration options, aliquoting, or custom fill volume for screening workflows.
- Documentation: construct map/sequence confirmation package (as available) and batch documentation.
What to include in your request
- Target cell type/model (cell line or primary cells) and intended readout (reporter, knockdown, OE, etc.)
- Insert sequence (FASTA) or reference ID, plus any required tags/mutations
- Promoter, reporter, and selection marker preferences
- Desired scale and preferred format (aliquots / concentration requests)
Email us at support@biohippo.com or use the Talk to a Scientist request form.
