| Field | Specification |
|---|---|
| Mfr No | |
| Accession Number | |
| Product Type | |
| Promoter | |
| Reporter | |
| Selection Marker | Puromycin, Blasticidin |
| Shipping | |
| Species |
Background
SREBP2 (sterol regulatory element-binding protein 2), encoded by SREBF2, is a membrane-bound basic helix-loop-helix leucine-zipper transcription factor that serves as the master regulator of cholesterol biosynthesis. When intracellular sterol levels fall, SREBP2 is escorted from the endoplasmic reticulum to the Golgi, where proteolytic cleavage releases its active N-terminal domain. This fragment enters the nucleus and binds sterol regulatory elements in the promoters of genes such as the LDL receptor and HMG-CoA reductase, driving cholesterol uptake and synthesis. SREBP2 activity is central to lipid homeostasis, and its dysregulation is linked to hypercholesterolemia, atherosclerosis, metabolic disease, and altered lipid metabolism in cancer.
Product Description & Applications
The SREBP2 Reporter Lentivirus is a lentiviral transcription-factor reporter system that provides a sensitive fluorescent or luminescent readout of SREBP2 transcriptional activity, useful for monitoring lipogenesis and sterol-regulated signaling. Tandem consensus sterol response elements are preferentially activated by SREBP2 and drive reporter gene expression, coupled to a minimal promoter optimized for signal-to-noise. The particles are purified by PEG precipitation and sucrose gradient centrifugation and a constitutively expressed selection marker (puromycin or blasticidin) supports stable reporter cell line generation. The system efficiently transduces difficult-to-transfect cells, including primary and thawed cultures, and supports readout by fluorescence microscopy, flow cytometry, or luminometry for studies of cholesterol metabolism, lipid homeostasis, and pathway-modulating compounds.
About This Product
This reporter lentivirus places a Firefly Luc, Renilla Luc, GFP, RFP, Luc reporter gene under the control of tandem consensus response elements specific for the Lipogenesis pathway (SREBP2) transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Puromycin, Blasticidin) and/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.
Stable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.
Can't find the lentiviral construct you need, or want to adjust key design elements? Contact us to discuss custom LV design and optional add-ons.
Common customization requests
- Insert / payload: replace the gene/sequence, swap to a different isoform, add mutations, or optimize cloning features.
- Expression design: change promoter (e.g., CMV/EF1α/PGK), add enhancers, or adjust regulatory elements.
- Reporters: add/swap GFP/RFP/mCherry/luciferase (single or dual reporters where applicable).
- Selection markers: add/swap puromycin/blasticidin/neomycin or fluorescent selection options.
- Vector format: switch between OE, shRNA, CRISPR (sgRNA/Cas systems), or control vectors (where supported).
Add-ons you can request
- Control viruses: empty vector, non-targeting shRNA, reporter-only controls, or matched backbone controls.
- Packaging / format: concentration options, aliquoting, or custom fill volume for screening workflows.
- Documentation: construct map/sequence confirmation package (as available) and batch documentation.
What to include in your request
- Target cell type/model (cell line or primary cells) and intended readout (reporter, knockdown, OE, etc.)
- Insert sequence (FASTA) or reference ID, plus any required tags/mutations
- Promoter, reporter, and selection marker preferences
- Desired scale and preferred format (aliquots / concentration requests)
Email us at support@biohippo.com or use the Talk to a Scientist request form.
