| Field | Specification |
|---|---|
| Target | |
| Alternative names | SRP72; YPL210CSignal recognition particle subunit SRP72; Signal recognition particle 72 kDa protein homolog |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | Recombinant Saccharomyces cerevisiae Signal recognition particle subunit SRP72 protein (1-180AA) |
| Conjugate | |
| Source | This product is a polyclonal antibody purified from rabbit antiserum. |
| Purification | |
| Form | Liquid |
| Storage | |
| Storage buffer | |
| Catalog no. (Mfr.) | |
| Main SKU |
Overview
This polyclonal anti-SRP72 antibody raised in Rabbit, tested in ELISA. Designed to detect SRP72 in Saccharomyces cerevisiae for others research.
Key elements and design rationale
- Immunogen: Recombinant Saccharomyces cerevisiae Signal recognition particle subunit SRP72 protein (1-180AA) — determines epitope; confirm sample prep compatibility.
- Host (Rabbit): Requires anti-rabbit-IgG secondary matching isotype IgG.
- Polyclonal: Multiple epitopes = robust signal; lot-to-lot variability — include controls.
- Isotype (IgG): Match secondary, Protein A/G, and isotype controls to IgG.
- Purification (Protein G purification): Enriches Ig classes; reduces non-specific background.
Biological background
SRP72 (also: SRP72, YPL210CSignal recognition particle subunit SRP72, Signal recognition particle 72 kDa protein homolog) — studied in Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) systems. Expression, localization, and PTMs vary across cell types and disease states. Consult UniProt, NCBI Gene, and primary literature for current SRP72 annotation relevant to others.
Common research applications
- ELISA: Quantify soluble target; ensure samples within the linear detection range.
Notes for experimental interpretation
- Isotype controls: IgG from Rabbit at equivalent concentration.
- Cross-reactivity: Polyclonal: validate with KO/KD controls.
- Matrix effects: Verify dilution linearity in your sample matrix.
- Reactivity: Confirmed for Saccharomyces cerevisiae; extrapolation requires validation.
This product is a polyclonal antibody purified from rabbit antiserum.
SRP72 is a protein studied in Saccharomyces cerevisiae biology. This polyclonal antibody raised in Rabbit detects SRP72 in ELISA applications, IgG isotype.
Reported reactive against Saccharomyces cerevisiae. Immunogen from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast). Cross-reactivity with untested species requires empirical confirmation.
Reported for ELISA. Optimize dilution/blocking per application. Consult datasheet for working concentrations.
Non-conjugated IgG; requires anti-rabbit-IgG secondary conjugated to your preferred reporter (HRP, AP, fluorophore, or biotin).
(1) Isotype control: IgG from Rabbit; (2) Positive control: lysate/cell line expressing SRP72; (3) Negative control: siRNA KD or KO; (4) Dilution linearity in your sample matrix.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
