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Description
ssDNA Assay Kit is a simple, sensitive, and accurate single-strand DNA (ssDNA) fluorescence quantitative detection kit with a good linear relationship between 1-200 ng. This kit contains a fluorescence detection reagent, buffer solution, and related ssDNA standards. Before use, dilute the buffer solution of fluorescence detection reagent into a working solution, and then add the ssDNA sample to be tested, then use the fluorescence microplate or Qubit Read with a fluorometer. The selectivity of this kit to single-stranded DNA is not higher than that to double-stranded DNA, but it has good tolerance to conventional pollutants such as proteins, salts, detergents, etc.
Features
- Fast and accurate ssDNA quantification
- High sensitivity, there is a good linear relationship in the low-concentration range
- Very suitable for the MGI platform library
Applications
- ssDNA quantification
- MGI Circular library and DNB quantification
- BS product quantification
Specifications
| Assay | ssDNA Quantitation |
| Excitation/Emission | 500/525 |
| For Use With (Equipment) | Qubit Fluorometer，fluorescence microplate |
| No. of Reactions | 100T/500T |
| Product Line | Qubit Quantitation |
| Quantitation Range | 1-200 ng, 50pg/μL - 200ng/μL |
| Sample Volume | 1 μL to 20 μL |
| Detection Method | Fluorescent |
Components
| Name | Concentration | 12646ES60 (100T) | 12646ES60 (500T) |
| ssDNA Reagent | 200× in DMSO | 250 μL | 1250 μL |
| ssDNA Buffer | Not applicable | 50 mL | 250 mL |
| ssDNA Standard 1 | 0 ng/μL in TE buffer | 1 mL | 5×1 mL |
| ssDNA Standard 2 | 20 ng/μL in TE buffer | 1 mL | 5×1 mL |
Shipping and Storage
All the components are shipped with ice packs and can be stored at 2℃~8℃ away from light for 6 months. Please avoid repeated freeze-thawing.
Figures
Figure 1 Linearity Performance
Test Sample: ssDNA standards; Conclusion: Good linearity within 0-200ng
Figure 2 Comparison of measured values of different batches and original Thermophile products
The stability of Yeasen（Cat # 12645）three batches of products is good, which can accurately quantify different types of ssDNA samples, and consistent with the competitive products(Thermo Q10212)
Documents:
This kit requires a fluorometer capable of detecting the kit's excitation/emission wavelength (typically ~480/520 nm for dsDNA HS and similar dyes). The Qubit fluorometer (Thermo Fisher) or compatible fluorometer-based systems are recommended. Standard UV-Vis spectrophotometers using A260 cannot substitute for fluorometric quantification when sample purity or volume is limiting.
Sensitivity varies by kit format: the HS (High Sensitivity) kit detects dsDNA as low as 0.2 ng/µL and the BR (Broad Range) kit covers 2–1000 ng/µL. Refer to the specific product datasheet for the validated linear range. Samples exceeding the range must be diluted before measurement.
Yes. The HS and BR dsDNA kits contain intercalating dyes with strong preference for double-stranded DNA over RNA and ssDNA. This selectivity makes them suitable for quantifying genomic DNA, PCR products, and library preparations in the presence of residual RNA without a DNase or RNase pre-treatment step.
Yes. Fluorometric dsDNA quantification is the recommended method for NGS library quantification, as it measures only double-stranded library fragments and is unaffected by free adapter dimers or unincorporated nucleotides (unlike A260). For highest accuracy on sequencing libraries, combine with Bioanalyzer or TapeStation sizing.
Yeasen Biotechnology offers flexible customization options for many of its assay kits and detection reagents, including custom lot sizes, bulk ordering, and application-specific formulation adjustments. Volume pricing, custom packaging, and kit bundling may be available depending on the product and intended workflow. A Certificate of Analysis (CoA) and lot-specific QC data are provided with every order. For inquiries regarding large-volume orders, custom configurations, or integration into automated workflows, please contact the BioHippo team for a tailored quotation.
