| Field | Specification |
|---|---|
| Applications | |
| Molecular weight | |
| Molecular formula | C30H36F12N12O10.1.5H2O |
| Purity | |
| SMILES | |
| Form | Solid |
| Storage | |
| Shipping | |
| Catalog no. (Mfr.) | |
| Main SKU |
Compound Overview
SSM3 tetraTFA hydrate is a potent furin inhibitor with an EC50 of 54 nM. It uses its positively charged guanidyl group to form strong electrostatic interactions with negatively charged residues in the catalytic center of furin, competitively occupying and blocking the active site, and it can be used in research on anthrax and avian influenza[1][2]. It is supplied as a white to off-white solid (C30H36F12N12O10.1.5H2O, MW 979.70) at 99.9% purity.
Physical & Chemical Properties
| Molecular Formula | C30H36F12N12O10.1.5H2O |
|---|---|
| Molecular Weight | 979.70 g/mol |
| Purity | 99.9% |
| Appearance | Solid |
| Color | White to off-white |
| SMILES | N=C(N)NC(C=C1)=CC=C1O[C@@H]2[C@H](C[C@@H](NC(N)=N)[C@H](OC3=CC=C(NC(N)=N)C=C3)C2)NC(N)=N.OC(C(F)(F)F)=O.OC(C(F)(F)F)=O.OC(C(F)(F)F)=O.OC(C(F)(F)F)=O.O.[1.5] |
| Signaling Pathway | Metabolic Enzyme/Protease |
| Solubility | In Vitro: DMSO: 30 mg/mL (30.62 mM; Requires sonication; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO) |
| Storage | -20°C, sealed storage, away from moisture. In solvent: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). |
| Shipping | Room temperature in continental US; may vary elsewhere. |
Literature Cited
Sources cited in this description and in the In Vitro & In Vivo Data tab. Peer-reviewed publications that used this product are listed under References.
Safety
For Research Use Only. Not for use in diagnostic or therapeutic procedures, and not for human or veterinary use. Handle in accordance with the Safety Data Sheet and your institution's chemical hygiene plan.
In Vitro
| Solvent | Solubility | Notes |
|---|---|---|
| DMSO | 30 mg/mL (30.62 mM) | requires sonication; use freshly opened DMSO (absorbed moisture lowers solubility) |
Aliquot the stock solution and store it at -80°C (up to 6 months) or -20°C (up to 1 month); sealed storage, away from moisture; avoid repeated freeze-thaw cycles.
Data provided by the manufacturer.
In Vitro
In the glioma cell line U251, SSM-3 (1-25 μM; 3 h) partly inhibits processing of PA83, the anthrax toxin protective antigen on the cell surface, into PA63[1]. In MCF7 breast cancer cells expressing an MT1-MMP mutant (MCF-MT1-E240A-FLAG cells), SSM-3 (10-100 μM; 18-24 h) blocks intracellular processing of pro-MT1-MMP into the mature enzyme, and in the fibrosarcoma cell line HT1080 it partly inhibits intracellular processing and release of TGFβ1[1]. SSM-3 is a potent inhibitor of purified human furin, with an EC50 of 54 nM[1]. At 100 μM, SSM-3 (0.01-100 μM; 24 h) is cytotoxic to mouse macrophage RAW 264.7 cells[1].
Western Blot Analysis[1]
| Cell Line | Human glioma U251 cells |
|---|---|
| Concentration | 1, 5, 25 μM |
| Incubation Time | 3 h |
| Result | Partially inhibited PA83 processing. |
Cell Cytotoxicity Assay[1]
| Cell Line | RAW 264.7 cell line |
|---|---|
| Concentration | 0.01, 0.1, 1, 10, 100 μM |
| Incubation Time | 24 h |
| Result | Exhibited cell toxicity at the 100 μM concentration. |
Western Blot Analysis[1]
| Cell Line | HT1080 cell line, MCF7 cell line (MCF-MT1-E240A-FLAG) |
|---|---|
| Concentration | 10, 100 μM |
| Incubation Time | 18, 24 h |
| Result | Blocked the intracellular processing of MT1-MMP. Partially suppressed the TGFβ1 processing and release. |
Data provided by the manufacturer. Numbered citations refer to the Literature Cited list in the product description.
Need this compound in a format that drops straight into your assay? We can tailor formulation, chemistry, and documentation so your results stay consistent across runs and re-orders.
- Format options: solid or pre-dissolved solution (choose solvent), target concentration, aliquots, light/moisture-protected packaging
- Chemistry options: free base/acid vs salt forms, hydrate/solvate preference, stereoisomer control (single enantiomer or racemate), close analogs
- Add-on labels & handles: D/¹³C/¹⁵N isotopes (LC-MS/internal standards), azide/alkyne or other functional handles for conjugation
- QC & documentation: standard COA or enhanced analytical pack (HPLC/LC-MS/NMR), chiral purity, residual solvents, water content (KF), method-specific specs
- Scale & continuity: mg to gram scale, bulk pricing, lot reservation, repeat-order continuity
To quote quickly, tell us: compound name + CAS/structure (SMILES or mol file), intended assay context, solvent preference, salt/stereochemistry requirements, purity/QC level, and the amount (mg–g).
Can’t find the compound you’re looking for?
Send the CAS or structure and your specs. We can help source it, suggest close equivalents, or discuss custom synthesis with the right QC documentation (RUO).
