ST cell

SKU:BHC11101302
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Overview
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ST cell is a cell line (Male). It is commonly used as an in vitro model for biosafety level 1. the cell line harbors porcine type-c oncovirus (pcov) sequences and their transcripts, and the possibility of viral secretion cannot be excluded. in germany, these viruses are categorized as bsl 1 for humans and bsl 2 for animals (trba 462). however, the german central committee on biological safety (zkbs) assigns a bsl 2 classification to these viruses and infected cell lines when used for genetic modification purposes. research. Growth characteristics: Adherent, Fibroblast. Supplied as cryopreserved cells with accompanying batch CoA and quality-control documentation.

Species Pig
Morphology Fibroblast
Growth Properties Adherent
Tissue Testis
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Catalog no. Size
305214 1 cryovial
Available Options

This cell line is available in the U.S. For non-profit users, please sign and submit the Non-Profit Supply Agreement to orders@biohippo.com before placing an order. For commercial users, please complete the CLEAR Form before ordering, as additional usage fees may apply based on the intended use. For further details, please contact orders@biohippo.com. Products ship after the required agreement is completed; typical delivery is 2–3 business days. Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10^6 cells for adherent lines or 5 × 10^6 cells for suspension lines (refer to the batch CoA for details).

Field Specification
Mfr No 305214
Species Pig
The ST cell line, derived from the connective tissue of a male Landrace pig, is primarily used in scientific studies related to virology and toxicology. These cells are of swine origin and are particularly valuable for research in veterinary medicine and comparative cellular biology, particularly for studies on viruses that affect swine. The fibroblast-like morphology of ST cells makes them a suitable model for studying cellular processes and virus-cell interactions in a porcine context. ST cells exhibit robust growth characteristics under standard cell culture conditions and have been utilized extensively to study a variety of swine pathogens, including foot-and-mouth disease virus and other Picornaviridae family members. Their susceptibility to different viral infections facilitates the analysis of viral life cycles, host-pathogen interactions, and the efficacy of antiviral compounds. Additionally, these cells are often used in the assessment of toxicological responses to various chemical agents, providing essential data on cellular responses and cytotoxicity in a non-human mammalian system. The versatility of the ST cell line in virological and toxicological assays underscores its utility in both fundamental and applied biological research. As such, ST cells continue to be a critical resource for researchers aiming to advance veterinary health, understand zoonotic disease mechanisms, and develop therapeutic strategies for diseases affecting swine populations.

SKU:BHC11101302

  • cultureMedium: EMEM (MEM Eagle), w: 2 mM L-Glutamine, w: 2.2 g/L NaHCO3, w: EBSS (Cytion article number 820100a)
  • supplements: Supplement the medium with 10% FBS, 1% NEAA and 1.0 mM Sodium pyruvate
  • dissociationReagent: Accutase
  • subculturing: Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
  • fluidRenewal: 2 to 3 times per week
  • freezeMedium: As a cryopreservation medium, use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
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Experience the power of Celltrypse™, c-LEcta's innovative enzyme solution for gentle and efficient cell dissociation. Request your free sample and discover a superior alternative for your cell culture workflows.

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