| Field | Specification |
|---|---|
| Mfr No | |
| Product Type | |
| Promoter | |
| Reporter | |
| Selection Marker | Blasticidin, Puromycin |
| Shipping | |
| Species |
Background
STAT4 (Signal Transducer and Activator of Transcription 4) is a latent cytoplasmic transcription factor of the JAK/STAT signaling pathway. In response to IL-12, STAT4 is phosphorylated by receptor-associated Janus kinases, dimerizes, and translocates to the nucleus to activate target genes. STAT4 is essential for the differentiation of naive CD4+ T cells into Th1 cells and for IFNγ production, and it contributes to IFNγ and IL-21 production in follicular helper T cells during antiviral responses. STAT4 also promotes natural killer cell antiviral activity by regulating Runx1 and Runx3. Because of its central role in cell-mediated immunity, STAT4 is an important target in immunology and inflammation research.
Product Description & Applications
The STAT4 Reporter Lentivirus is a transcription-factor reporter system that provides a sensitive fluorescent or luminescent readout of STAT4 activity in mammalian cells. It uses tandem repeats of STAT4 responsive elements from the human T-bet enhancer, with optimized flanking nucleotides that bias the readout toward STAT4 over the related STAT1, so the reporter responds preferentially to IL-12 signaling. A constitutively expressed selection marker and optional secondary reporter enable stable polyclonal reporter cell lines.
Stable lentiviral integration gives consistent reporter expression in dividing and post-mitotic cells, including primary and thawed cultures, avoiding transient-transfection variability. Particles are purified by PEG precipitation and sucrose gradient centrifugation. The system is used to study STAT4 activity and JAK/STAT signaling, with readout by fluorescence microscopy, flow cytometry, or luminometry.
About This Product
This reporter lentivirus places a d2GFP, EGFP, Firefly Luc, GFP, Luc, mCherry, RFP reporter gene under the control of tandem consensus response elements specific for the STAT4 transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Puromycin) and/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.
Stable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.
Can't find the lentiviral construct you need, or want to adjust key design elements? Contact us to discuss custom LV design and optional add-ons.
Common customization requests
- Insert / payload: replace the gene/sequence, swap to a different isoform, add mutations, or optimize cloning features.
- Expression design: change promoter (e.g., CMV/EF1α/PGK), add enhancers, or adjust regulatory elements.
- Reporters: add/swap GFP/RFP/mCherry/luciferase (single or dual reporters where applicable).
- Selection markers: add/swap puromycin/blasticidin/neomycin or fluorescent selection options.
- Vector format: switch between OE, shRNA, CRISPR (sgRNA/Cas systems), or control vectors (where supported).
Add-ons you can request
- Control viruses: empty vector, non-targeting shRNA, reporter-only controls, or matched backbone controls.
- Packaging / format: concentration options, aliquoting, or custom fill volume for screening workflows.
- Documentation: construct map/sequence confirmation package (as available) and batch documentation.
What to include in your request
- Target cell type/model (cell line or primary cells) and intended readout (reporter, knockdown, OE, etc.)
- Insert sequence (FASTA) or reference ID, plus any required tags/mutations
- Promoter, reporter, and selection marker preferences
- Desired scale and preferred format (aliquots / concentration requests)
Email us at support@biohippo.com or use the Talk to a Scientist request form.
