| Field | Specification |
|---|---|
| Mfr No | |
| Product Type | |
| Promoter | |
| Reporter | |
| Selection Marker | Blasticidin, Puromycin |
| Shipping | |
| Species |
Background
STAT5 comprises two closely related transcription factors, STAT5A and STAT5B, that act as latent cytoplasmic effectors of the JAK/STAT signaling pathway. In response to cytokines and growth factors such as interleukins, prolactin, growth hormone, and erythropoietin, receptor-associated Janus kinases phosphorylate STAT5, which then dimerizes, enters the nucleus, and binds specific response elements to drive transcription. STAT5 target genes regulate cell proliferation, survival, and differentiation, and the pathway is essential for hematopoiesis and immune cell development. Constitutive STAT5 activation is associated with leukemias and other malignancies, making STAT5 an important node in cytokine signaling and cancer research.
Product Description & Applications
The STAT5 Reporter Lentivirus is a transcription factor reporter system that provides a sensitive fluorescent or luminescent readout of STAT5 activity in human or mouse cells. Tandem consensus STAT5 response elements drive expression of a reporter (GFP, RFP, firefly luciferase, Renilla luciferase, or SEAP), enabling quantitative monitoring of JAK/STAT pathway activation in transduced cells. Antibiotic selection markers (puromycin or blasticidin) support establishment of stable reporter cell lines. The system is used to study cytokine and growth factor signaling, characterize hematopoietic and immune responses, and screen pathway modulators. Supplied as lentiviral particles purified by PEG precipitation and sucrose gradient centrifugation, it efficiently transduces difficult-to-transfect cells, including primary and thawed cells.
About This Product
This reporter lentivirus places a Firefly Luc, GFP, Luc, Renilla Luc, RFP, SEAP reporter gene under the control of tandem consensus response elements specific for the STAT5 signaling pathway transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Puromycin) and/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.
Stable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.
Can't find the lentiviral construct you need, or want to adjust key design elements? Contact us to discuss custom LV design and optional add-ons.
Common customization requests
- Insert / payload: replace the gene/sequence, swap to a different isoform, add mutations, or optimize cloning features.
- Expression design: change promoter (e.g., CMV/EF1α/PGK), add enhancers, or adjust regulatory elements.
- Reporters: add/swap GFP/RFP/mCherry/luciferase (single or dual reporters where applicable).
- Selection markers: add/swap puromycin/blasticidin/neomycin or fluorescent selection options.
- Vector format: switch between OE, shRNA, CRISPR (sgRNA/Cas systems), or control vectors (where supported).
Add-ons you can request
- Control viruses: empty vector, non-targeting shRNA, reporter-only controls, or matched backbone controls.
- Packaging / format: concentration options, aliquoting, or custom fill volume for screening workflows.
- Documentation: construct map/sequence confirmation package (as available) and batch documentation.
What to include in your request
- Target cell type/model (cell line or primary cells) and intended readout (reporter, knockdown, OE, etc.)
- Insert sequence (FASTA) or reference ID, plus any required tags/mutations
- Promoter, reporter, and selection marker preferences
- Desired scale and preferred format (aliquots / concentration requests)
Email us at support@biohippo.com or use the Talk to a Scientist request form.
