| Field | Specification |
|---|---|
| Target | |
| Alternative names | STAT5A; STAT5; Signal transducer and activator of transcription 5A |
| Gene ID | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human STAT5A around the phosphorylation site of Ser780. AA range:745-794 |
| Conjugate | |
| Concentration | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Scientific Background
The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of this protein in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for tumorigenesis. The mouse counterpart of this gene is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of this gene in cells. Alternatively spliced transcript variants have been
Product Overview
This polyclonal antibody, raised in Rabbit, recognizes Stat5a (phospho Ser780). It is validated for Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation, ELISA.
Validated Applications
| Applications | Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation, ELISA |
|---|---|
| Western Blot | 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunoprecipitation: 2-5 ug/mg lysate. ELISA: 1/10000. Not yet tested in other applications. |
Optimal dilutions should be determined by the end user for each application and sample type.
Immunogen
The antiserum was produced against synthesized peptide derived from human STAT5A around the phosphorylation site of Ser780. AA range:745-794
Specifications
| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Immunogen | The antiserum was produced against synthesized peptide derived from human STAT5A around the phosphorylation site of Ser780. AA range:745-794 |
| Concentration | 1 mg/mL |
| Observed Band | 91kD |
| Subcellular Location | Cytoplasm . Nucleus . Translocated into the nucleus in response to phosphorylation. |
| Swiss-Prot | P42229 |
| Gene ID | 6776 |
| Storage | -20°C/1 year |
Reactivity Notes
Validated reactivity: Human, Mouse, Rat. For other species, epitope conservation may allow cross-reactivity; confirm with appropriate controls.
Safety & Handling
This product is for research use only (RUO) and is not intended for diagnostic or therapeutic use. The formulation contains sodium azide; handle with standard laboratory precautions.
Related Products
Explore related antibodies and reagents for Stat5a (phospho Ser780) research from ELK Biotechnology, or contact us for alternative formats.
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