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Product Overview
This kit is suitable for separating total DNA from 180–220 mg of fresh or frozen human or animal feces. The genomic DNA, viral DNA, bacteria and parasite DNA of the human or animal in the dissolved feces can be bound to the nucleic acid purification column, and degraded protein and PCR inhibitors are removed by the Solution InR precipitation and the column wash steps, and the genomic DNA is washed with Solution RP and Wash Buffer. After washing, it can be used in various molecular biology experiments by eluting with the supplied Elution Buffer.
The sample is lysed in 1.4 mL of Solution SGE in a 95°C water bath for 5 min, clarified with one fifth volume of Solution InR, then digested with 10 µL of Proteinase K and 200 µL of Solution GA2 in a 70°C water bath for 10 min. After adding 200 µL of absolute ethanol the mixture is loaded onto Adsorption column G, washed with 500 µL of Solution RP and 700 µL of Wash Buffer, dried by centrifugation at 12,000 rpm for 2 min, and eluted with 50–200 µL of Elution Buffer after 2–5 min at room temperature.
Specifications
| Format | Spin column (Adsorption column G) |
|---|---|
| Sample types | Fresh or frozen human or animal feces, solid or liquid |
| Sample input | 180–220 mg feces, or 200 µL of liquid feces |
| Nucleic acids recovered | Genomic, viral, bacterial and parasite DNA |
| Processing time | Within 1 hour |
| Elution volume | 50–200 µL |
Kit Components
| Component | 50 T | 200 T |
|---|---|---|
| Solution SGE | 80 mL | 160 mL × 2 |
| Solution InR | 15 mL | 60 mL |
| Solution GA2 | 12 mL | 48 mL |
| Wash Buffer | 70 mL | 280 mL |
| Solution RP | 25 mL | 100 mL |
| Elution Buffer | 10 mL | 40 mL |
| ProteinaseK | 525 µL | 2.1 mL |
| Adsorption column G | 50 set | 200 set |
Applications
- Total DNA extraction from human or animal feces
- Recovery of bacterial and parasite DNA from stool
- Recovery of viral DNA from stool
- Downstream molecular biology experiments on stool DNA
Key Features
- Wide range of applications: Suitable for solid or liquid fecal samples from different sources.
- Simple and fast: High purity genomic DNA can be obtained within 1 hour.
- High purity: The high-efficiency precipitant removes impurities such as humic acid and can be directly used in downstream experiments.
Storage & Handling
All components are stored at room temperature for 1 year, except Proteinase K, which is stored at -20°C for 1 year.
Usage Notes
- Store freshly collected fecal samples at -20°C or lower without delay; 2–3 hours at room temperature causes degradation and longer storage risks severe DNA degradation.
- Repeating the Wash Buffer step increases purity.
- Remove residual ethanol by centrifuging at 12,000 rpm for 2 min and air-drying the column, to avoid inhibition of downstream enzymes.
- For sensitive assays, elute with sterile water at pH 7.0–8.5 instead of Elution Buffer.
- A second elution with a further 50–200 µL increases yield.
- Store the eluted DNA at -20°C.
Safety & Regulatory
For research use only. Not intended for clinical, diagnostic or therapeutic use. Follow the manufacturer's manual and standard laboratory safety practice.
Customization & Add-ons: Need this molecular biology reagent tailored to your workflow? We can help with bulk or custom pack sizes, alternative formats (spin column, magnetic bead or reagent-only kits; premixed or stand-alone reference dye), matched extraction, cDNA synthesis and qPCR workflows, and sourcing of related enzymes, ladders and consumables not in our catalog. Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services — our team will follow up with feasibility details and next steps.
Effects of sodium butyrate supplementation on inflammation, gut microbiota, and short-chain fatty acids in Helicobacter pylori-infected mice. Helicobacter, 2021. 10.1111/hel.12785
Comparative modulation of the gut microbiota by date-derived products and refined sugar: a 16S rRNA gene taxonomic profile in healthy rats. Frontiers in Microbiology, 2026. 10.3389/fmicb.2026.1762458
