| Field | Specification |
|---|---|
| Mfr No | |
| Product Type | |
| Shipping | |
| Source | Recombinant (E. coli) |
| Storage |
SUMO protease recognizes the complete SUMO (Small Ubiquitin-like Modifier) label protein with 100 amino acids and efficiently cuts the SUMO from the fusion protein. Compared with the recognition sites of EK and TEV, SUMO protease has high specificity due to its long recognition sequence, and maintains high activity in a wide range of reaction environmental systems, such as temperature (4-30℃), pH (5.5-9.5), etc. SUMO protease also has a polymerized His label, which is convenient to remove SUMO protease and purify the target protein by affinity chromatography.
Specifications
|
Cat.No. |
20410ES60 / 20410ES70 / 20410ES80 |
|
Size |
100 U / 500 U / 1000 U |
|
Enzyme activity |
1 U/μL |
|
Unit Definition |
The amount of enzyme required to cut >85% of the 3 μg substrate in a 1×rTEV Buffer (50 mM Tris, pH 8.0, 0.1 mM EDTA, 1 mM DTT) at 30℃ for 1 h was defined as a unit of activity |
|
Source |
Expressed in E. coli |
|
Label |
Polymerized His label |
|
Protein purity |
> 90% |
|
Molecular weight |
26.55 kDa |
|
Storage Buffer |
25 mM Tris-HCl, pH 8.0、0.1% Igepal (NP-40)、250 mM NaCl、500 μM DTT、50% glycerin |
Glycerol Content |
Contains Glycerol |
Components
Note：The enzyme activity definition uses 10×Protease Buffer (-salt). SUMO Protease has relatively high activity in 10×Protease Buffer (-salt), but for unstable substrates, 10×Protease Buffer (+salt) should be selected. We also provide reaction buffer 20410-B2 with +salt. Choose the correct buffer for your experiment. 20410-B2：500 mM Tris-HCl, pH 8.0, 2% Igepal (NP-40), 1.5 M NaCl, 10 mM DTT；20410-B1：500 mM Tris-HCl, pH 8.0, 2% Igepal (NP-40), 10 mM DTT. |
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Storage
This product should be stored at -85~-65℃ for 1 year.
Instructions
- The following reaction system is formulated in the EP tube:
- Incubate at 30℃, suck out 20 μL of the above reaction solution at 1, 2, 4 and 6 hours in a separate EP tube respectively.
- Add 20 μL of 2×SDS loading Buffer into the EP tube and place it at -20℃.
- After the reaction of all samples was completed, the samples were boiled for 5 min and 30 μL was taken for SDS-PAGE analysis. Determine the optimal response time.
|
Components |
Volume (μL) (The reaction system can be scaled down equally) |
|
Fusion protein |
50 μg |
|
10×Protease Buffer |
20 μL |
|
SUMO Protease (1U/μL) |
10 μL |
|
ddH2O |
to 200 μL |
Note: If the fusion protein requires low temperature treatment, the reaction solution can be placed at 4℃ to prolong the reaction time and increase the amount of SUMO enzyme to ensure the effect of enzyme digestion.
Table 1. SUMO Protease digestion activity at different temperatures
|
Incubation Time（h） |
Digestion activity at different temperatures (%) |
|||
|
4℃ |
16℃ |
25℃ |
30℃ |
|
|
0.5 |
48 |
73 |
83 |
88 |
|
1 |
60 |
87 |
90 |
93 |
|
2 |
71 |
94 |
94 |
95 |
|
3 |
74 |
95 |
95 |
95 |
Notes
1. Please wear the necessary PPE, such lab coat and gloves, to ensure your health and safety.
2. This product is for research use only.
Store this enzyme at -20°C and avoid repeated freeze-thaw cycles to preserve catalytic activity. The product is shipped Dry Ice and remains stable for up to one year from the date of manufacture when stored under recommended conditions. Aliquoting the stock solution into single-use volumes is recommended for enzymes used infrequently to minimize thermal cycling of the bulk stock.
This enzyme is validated for use in Protein Research, Nucleic Acid Purification applications. It is suitable for use in both standard laboratory research settings and, where applicable, optimized reaction workflows requiring high specificity, sensitivity, or throughput.
One unit (U/mg) is defined as the amount of protease activity per milligram of enzyme protein under standardized assay conditions (substrate concentration, pH, temperature, and incubation time).
This enzyme is produced as Recombinant (E. coli) and supplied as a Research Use Only (RUO) reagent. Each lot is subjected to activity assay, purity assessment by SDS-PAGE, and functional validation prior to release. A Certificate of Analysis (CoA) and Safety Data Sheet (SDS) are available on request.
Most serine proteases (e.g., Proteinase K) are inhibited by PMSF (1 mM) and heat-inactivated at 95°C for 10 min. Proteinase K retains activity across a broad pH range (4–12) and tolerates SDS, urea, and other denaturants, making it particularly effective for nucleic acid extraction from challenging samples including FFPE tissue.
Yeasen Biotechnology supports custom enzyme solutions across multiple service lines — from GMP-grade bulk supply to directed enzyme engineering. Contact BioHippo to discuss requirements and initiate a project inquiry.
▶ GMP-Grade & Bulk Supply
Select Yeasen enzymes are available in GMP grade, manufactured in an ISO 13485-certified UCF.ME™ ultra-clean molecular enzyme facility with FDA Drug Master File (DMF) support.
- GMP-grade release testing and CoA documentation
- ISO 13485-certified production facility
- Scalable from milligram to multi-gram quantities
- Consistent lot-to-lot activity specifications
▶ Glycerol-Free & Custom Formulation
Glycerol-free enzyme formats are available for applications requiring lyophilization compatibility, liquid handling automation, or direct IVD master mix integration.
- Glycerol-free liquid format (standard and custom buffers)
- Lyophilization-ready enzyme preparation
- Custom reaction buffer optimization for specific assay conditions
- Compatible with freeze-drying workflows for point-of-care formats
▶ Molecular IVD RDC Service
Yeasen's Research and Development Contracting (RDC) team delivers end-to-end solutions for molecular diagnostic product development, covering enzyme selection through clinical validation support.
- Enzyme selection and performance matching
- Primer/probe design and reaction buffer optimization
- Sensitivity, specificity, and precision validation studies
- Stability studies and SNP evaluation
- Instrument platform compatibility assessment
▶ ZymeEditor™ Enzyme Engineering
Yeasen's proprietary ZymeEditor™ directed evolution and rational design platform enables the development of custom enzyme variants with tailored performance characteristics not available in off-the-shelf products.
- Directed evolution for enhanced thermostability, processivity, or fidelity
- Rational design for altered substrate specificity or cofactor requirements
- Library screening from Yeasen's proprietary enzyme variant collection
- Scale-up to commercial quantities upon candidate confirmation
ⓘ Customization services are fulfilled by Yeasen Biotechnology. Lead times and minimum order quantities vary by service type. Contact BioHippo for project scoping and pricing.
