Supercoiled Plasmid DNA

What it means: 1 mg/ml — the concentration of the supplied stock.

Why it matters:

• The stock concentration decides how far you dilute the product for each reaction.
• Knowing it lets you work out how many reactions one vial supports.
• Using the same final amount in every reaction keeps results comparable.
• A stated concentration makes dilution steps easy to record and repeat.
• It also helps you compare your conditions with published methods.
• Writing down the lot and stock concentration helps when you compare runs over time.
• Over-concentrated working solutions can raise background or waste material.

For this product: The DNA is supplied at 1 mg/ml in both sizes, so the same dilution works for either option.

Tip: Mix gently before taking an aliquot; avoid vortexing plasmid DNA hard.

What it means: 0.050 mg or 1 mg — how much product comes in one unit.

Why it matters:

• Pack size helps you plan how many experiments one order covers.
• It lets you set aside material for repeats and controls from the same lot.
• Knowing the amount helps you schedule reorders before you run out.
• It makes it easy to check the shipment against the order on arrival.
• Smaller sizes suit pilot work, while larger sizes cover a full study.
• The amount per unit also decides how often a vial is opened and handled.
• Ordering the right size reduces waste from material that is not used in time.

For this product: Choose 0.050 mg or 1 mg; both sizes contain the same DNA at the same concentration.

Tip: Use the 0.050 mg size for pilot runs and the 1 mg size for screening campaigns.

What it means: TE buffer — the buffer the product is supplied in.

Why it matters:

• The buffer is carried into your reaction when you add the product.
• Knowing its components helps you check compatibility with your assay buffer.
• EDTA and salts in the buffer can matter for metal-dependent enzymes.
• Accounting for the added buffer keeps final reaction conditions as planned.
• Stating the buffer makes your methods easy to report and repeat.
• Buffer components can affect enzyme activity if their final concentration is high.
• Keeping the same buffer in all reactions makes results comparable.

For this product: The DNA is supplied in TE buffer; the EDTA it contains is carried into your reaction with the DNA.

Tip: Account for the added TE buffer when you set the final magnesium concentration of the reaction.

What it means: Frozen — how the product is shipped.

Why it matters:

• The shipping condition tells you what to expect when the package arrives.
• Unpacking promptly lets you check the contents against the order.
• Checking the package on arrival helps you spot transport problems early.
• Storage conditions can differ from shipping conditions, so confirm them before the package arrives.
• Recording the arrival date and condition helps if questions come up later.
• Arranging for someone to receive the package avoids delays when it arrives.
• The shipping condition is chosen to keep the product as described during transport.

For this product: Ships frozen; unpack it promptly on arrival. Please contact us to confirm storage conditions.

Tip: Open and check the package on arrival, and contact us right away if anything is damaged or missing.