| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human SYNJ2 recombinant protein (Position: D18-K1495) was used as the immunogen for the SYNJ2 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
SYNJ2 Antibody / Synaptojanin 2 is a anti-SYNJ2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Flow cytometry (FACS), ELISA with listed reactivity in Human. Reported localization: Cytoplasm, cell membrane.
Key elements and design rationale
- Target: SYNJ2
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, FACS, ELISA
Biological background
SYNJ2 is expressed in many tissues, with particularly high levels in the brain, liver, and kidney. It localizes to endocytic vesicles, the plasma membrane, and cytoplasmic puncta associated with actin filaments. Through its phosphatase activity, SYNJ2 terminates phosphoinositide-dependent signaling, facilitating the disassembly of endocytic complexes. It interacts with endophilins, amphiphysin, and dynamin to coordinate vesicle fission and cargo retrieval. In addition, SYNJ2 participates in insulin signaling and receptor recycling in adipocytes and hepatocytes, linking membrane trafficking to metabolic regulation.
The SYNJ2 antibody is widely used in neuroscience, cell biology, and cancer research to study phosphoinositide metabolism and endocytosis. Western blot analysis typically identifies a 170 kilodalton band corresponding to full-length Synaptojanin-2, while immunofluorescence reveals punctate staining along endocytic vesicles and actin-rich regions. Dysregulated SYNJ2 expression has been associated with enhanced tumor cell migration and invasion, particularly in breast and prostate cancers, where it drives actin remodeling and lamellipodia formation through PI3K signaling modulation.
Functionally, SYNJ2 acts at the intersection of signaling and membrane dynamics, ensuring timely lipid turnover and vesicle recycling. It also contributes to synaptic vesicle endocytosis, influencing neuronal plasticity and receptor availability. The SYNJ2 antibody supports these studies by providing precise detection of Synaptojanin-2 across multiple tissue types.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
