| Field | Specification |
|---|---|
| Target | |
| Alternative names | Synaptotagmin-1|Synaptotagmin I (SytI)|p65|SYT1|SVP65|SYT |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | synaptotagmin I |
| Conjugate | |
| Molecular weight | |
| Purity | |
| Purification | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Scientific Background
The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis(PMID: 8058779). SYT1(synaptotagmin I) was firstly identified as a 65-kDa protein with a wide distribution in neuronal and neurosecretory tissue(PMID: 7298720). Calcium binding to SYT1 participates in triggering neurotransmitter release at the synapse(PMID: 11242035). In addition to regulating exocytosis, SYT1 has also been implicated in endocytosis and neurite outgrowth(PMID: 15492212; 11078930; 10336114). This antibody recognizes endogenous SYT1, which has an experimentally determined molecular mass of 65-68 kDa. An additional band of 47 kDa could also be detected, which is corresponding to the calculated molecular weight of SYT1.
Product Overview
This polyclonal antibody, raised in Rabbit, recognizes SYT1. It is validated for ELISA, Western Blot, Immunohistochemistry, Immunoprecipitation.
Validated Applications
| Applications | ELISA, Western Blot, Immunohistochemistry, Immunoprecipitation |
|---|---|
| WB | 1:500-1:1000 |
| IP | 1:500-1:1000 |
| IHC | 1:20-1:200 |
| IF | 1:50-1:500 |
Optimal dilutions should be determined by the end user for each application and sample type.
Immunogen
synaptotagmin I
Specifications
| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Immunogen | synaptotagmin I |
| Purification | Immunogen Affinity Purified |
| Purity | ≥95% as determined by SDS-PAGE |
| Form | Liquid |
| Target MW | 65 kDa |
| UniProt # | P21579 |
| Storage | PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.) |
| Alternative Names | Synaptotagmin-1|Synaptotagmin I (SytI)|p65|SYT1|SVP65|SYT |
Reactivity Notes
Validated reactivity: Human, Mouse, Rat. For other species, epitope conservation may allow cross-reactivity; confirm with appropriate controls.
Safety & Handling
This product is for research use only (RUO) and is not intended for diagnostic or therapeutic use. The formulation contains sodium azide; handle with standard laboratory precautions.
Related Products
Explore related antibodies and reagents for SYT1 research from Fine Test, or contact us for alternative formats.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
