| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthesized peptide derived from human SYVN1 / HRD1 was used as the immunogen for the SYVN1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
SYVN1 Antibody / Synoviolin / HRD1 is a anti-SYVN1 Rabbit antibody Recombinant Rabbit Monoclonal clone 32S38 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow cytometry (FACS) with listed reactivity in Human, Mouse, Rat. Reported localization: ER.
Key elements and design rationale
- Target: SYVN1
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 32S38, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB, ICC, IF, IP, FACS
Biological background
ER-associated degradation is essential for secretory pathway fidelity. Research using SYVN1 antibody has shown that Synoviolin forms part of the ER membrane-anchored ubiquitin ligase complex, recognizing unfolded substrates and ubiquitinating them for retrotranslocation and proteasomal degradation. This prevents accumulation of misfolded proteins, protecting cells from ER stress and apoptosis. By maintaining proteostasis, SYVN1 safeguards secretory and metabolic tissues.
Aberrant expression of Synoviolin has been linked to cancer, fibrosis, and autoimmune disease. Studies with SYVN1 antibody have revealed that overexpression promotes tumor survival by reducing ER stress-induced apoptosis. In rheumatoid arthritis, Synoviolin overexpression in synovial tissue contributes to pathological proliferation and joint destruction. These findings highlight SYVN1 as both a biomarker and a potential therapeutic target.
Beyond pathology, SYVN1 contributes to normal development and physiology. Research using SYVN1 antibody has shown that knockout mice exhibit embryonic lethality due to defective ERAD, confirming its essential function. In the liver, SYVN1 regulates lipid metabolism and detoxification processes by controlling ER protein load. These functions underscore the importance of Synoviolin in both health and disease.
SYVN1 antibody is widely used in western blotting, immunohistochemistry, and immunoprecipitation. Western blotting confirms expression in liver and synovium, immunohistochemistry highlights tissue-specific overexpression in disease, and immunoprecipitation identifies substrates ubiquitinated by Synoviolin. These methods make SYVN1 antibody a versatile tool in cell biology and translational research.
By supplying validated SYVN1 antibody reagents,
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
