| Field | Specification |
|---|---|
| Mfr No | |
| Product Type | |
| Promoter | |
| Reporter | |
| Selection Marker | Blasticidin, Puromycin |
| Shipping | |
| Species |
Background
T-bet (TBX21) is a T-box transcription factor that serves as the master regulator of type 1 immunity. It directs the differentiation of naive CD4+ T cells toward the Th1 lineage, driving expression of interferon-gamma and other effector genes, and also shapes the function of CD8+ T cells, natural killer cells, and dendritic cells. In B lymphocytes, T-bet contributes to immunoglobulin class switching. T-bet binds DNA through a conserved T-box motif, and the T-half site is recognized by other T-box proteins whose distinct optimal sequences confer target gene specificity. Because T-bet activity determines the balance between Th1 and Th2 responses, it is central to research on infection, autoimmunity, and inflammation.
Product Description & Applications
The T-bet Reporter Lentivirus is a transcription-factor reporter system that provides a sensitive fluorescent or luminescent readout of human or mouse T-bet transcriptional activity. The construct places a reporter gene (fluorescent or luciferase) under the control of tandem T-bet response elements coupled to a minimal promoter, while a constitutively expressed selection marker supports stable cell line generation. Following transduction and stable integration, reporter output can be measured by fluorescence microscopy, flow cytometry, or luminometry.
The system is well suited to dissecting T-bet signaling and Th1 lineage commitment, screening pathway modulators, and studying immune responses. Particles are purified by PEG precipitation and sucrose gradient centrifugation, making them effective in difficult-to-transfect cells including primary and cryopreserved cells.
About This Product
This reporter lentivirus places a d2GFP, EGFP, Firefly Luc, GFP, mCherry, RFP reporter gene under the control of tandem consensus response elements specific for the T-bet transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Puromycin) and/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.
Stable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.
Can't find the lentiviral construct you need, or want to adjust key design elements? Contact us to discuss custom LV design and optional add-ons.
Common customization requests
- Insert / payload: replace the gene/sequence, swap to a different isoform, add mutations, or optimize cloning features.
- Expression design: change promoter (e.g., CMV/EF1α/PGK), add enhancers, or adjust regulatory elements.
- Reporters: add/swap GFP/RFP/mCherry/luciferase (single or dual reporters where applicable).
- Selection markers: add/swap puromycin/blasticidin/neomycin or fluorescent selection options.
- Vector format: switch between OE, shRNA, CRISPR (sgRNA/Cas systems), or control vectors (where supported).
Add-ons you can request
- Control viruses: empty vector, non-targeting shRNA, reporter-only controls, or matched backbone controls.
- Packaging / format: concentration options, aliquoting, or custom fill volume for screening workflows.
- Documentation: construct map/sequence confirmation package (as available) and batch documentation.
What to include in your request
- Target cell type/model (cell line or primary cells) and intended readout (reporter, knockdown, OE, etc.)
- Insert sequence (FASTA) or reference ID, plus any required tags/mutations
- Promoter, reporter, and selection marker preferences
- Desired scale and preferred format (aliquots / concentration requests)
Email us at support@biohippo.com or use the Talk to a Scientist request form.
