| Field | Specification |
|---|---|
| Mfr No | |
| Product Type | |
| Reporter | |
| Selection Marker | Hygromycin, Zeocin, Puromycin, Blasticidin |
| Shipping | |
| Species |
Background
T-Pit (TBX19) and PitX1 are transcription factors that act together to specify and maintain the corticotrope and melanotrope lineages of the pituitary gland. T-Pit is a T-box factor essential for terminal differentiation of pro-opiomelanocortin (POMC)-expressing cells, while PitX1 is a paired-like homeodomain factor with broad roles in pituitary and craniofacial development. The two factors bind composite CE3 response elements and synergistically activate transcription of POMC, the precursor of ACTH and other peptide hormones, and of corticotropin-releasing hormone signaling targets. Loss of T-Pit function causes isolated ACTH deficiency, making this transcription factor pair central to neuroendocrine and pituitary developmental research.
Product Description & Applications
The T-Pit/PitX1 Reporter Lentivirus is a transcription-factor reporter system for detecting the synergistic transcriptional activity of T-Pit and PitX1 in mammalian cells. The construct uses tandem repeats of composite CE3 response elements that bind both factors, coupled to a minimal promoter that drives a fluorescent or luminescent reporter, providing a readout reflecting their cooperative activation of POMC and corticotropin-related genes. A constitutively expressed selection marker and optional secondary reporter support stable polyclonal reporter cell lines.
Stable lentiviral integration provides consistent reporter expression in dividing and post-mitotic cells, including primary and cryopreserved cultures, avoiding transient-transfection variability. Particles are purified by PEG precipitation and sucrose gradient centrifugation and transduce difficult-to-transfect cells, supporting research on corticotrope differentiation and pituitary development.
About This Product
This reporter lentivirus places a BFP2, d2GFP, EGFP, Firefly Luc, Gaussia Luc, GFP, GFP + Firefly Luc, mCherry, Renilla Luc, RFP, RFP + Firefly Luc reporter gene under the control of tandem consensus response elements specific for the T-Pit & PitX1 transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Hygromycin, Zeocin, Puromycin, Blasticidin) and/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.
Stable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.
Can't find the lentiviral construct you need, or want to adjust key design elements? Contact us to discuss custom LV design and optional add-ons.
Common customization requests
- Insert / payload: replace the gene/sequence, swap to a different isoform, add mutations, or optimize cloning features.
- Expression design: change promoter (e.g., CMV/EF1α/PGK), add enhancers, or adjust regulatory elements.
- Reporters: add/swap GFP/RFP/mCherry/luciferase (single or dual reporters where applicable).
- Selection markers: add/swap puromycin/blasticidin/neomycin or fluorescent selection options.
- Vector format: switch between OE, shRNA, CRISPR (sgRNA/Cas systems), or control vectors (where supported).
Add-ons you can request
- Control viruses: empty vector, non-targeting shRNA, reporter-only controls, or matched backbone controls.
- Packaging / format: concentration options, aliquoting, or custom fill volume for screening workflows.
- Documentation: construct map/sequence confirmation package (as available) and batch documentation.
What to include in your request
- Target cell type/model (cell line or primary cells) and intended readout (reporter, knockdown, OE, etc.)
- Insert sequence (FASTA) or reference ID, plus any required tags/mutations
- Promoter, reporter, and selection marker preferences
- Desired scale and preferred format (aliquots / concentration requests)
Email us at support@biohippo.com or use the Talk to a Scientist request form.
