| Field | Specification |
|---|---|
| Mfr No | |
| Product Type | |
| Shipping | |
| Source | Recombinant (E. coli) |
| Storage |
T4 DNA Polymerase catalyzes the synthesis of DNA in the 5'→3' direction and requires the presence of a template and a primer. This enzyme has a 3'→5' exonuclease activity, but it does not have a 5'→3' exonuclease function.
Applications
3´ overhang removal to form blunt ends
5´ overhang fill-in to form blunt ends
Second strand synthesis in site-directed mutagenesis
Probe labeling using replacement synthesis
Cloning of PCR products without reliance on ligation reactions
Specifications
|
Source |
Bacteriophage T4 |
|
Molecular Weight |
103 kDa |
|
Concentration |
5 U/μL |
|
Unit Definition |
One unit is defined as the amount of enzyme required to catalyze the incorporation of 10 nmol of deoxyribonucleotide triphosphates (dNTPs) into a polynucleotide within 30 minutes at 37°C |
Glycerol Content |
Contains Glycerol |
Components
|
Name |
12901ES60 |
12901ES76 |
12901ES90 |
12901ES08 |
|
|
12901-A |
T4 DNA Polymerase(5 U/μL) |
20 μL |
100 μL |
400 μL |
1 mL |
|
12901-B |
10 × Blue Buffer |
100 μL |
250 μL |
500 μL |
1.25 mL |
Shipping and Storage
This product should be stored at -25 ~ -15℃ for 1 year.
Store this enzyme at -20°C and avoid repeated freeze-thaw cycles to preserve catalytic activity. The product is shipped Dry Ice and remains stable for up to one year from the date of manufacture when stored under recommended conditions. Aliquoting the stock solution into single-use volumes is recommended for enzymes used infrequently to minimize thermal cycling of the bulk stock.
3´ overhang removal to form blunt ends 5´ overhang fill-in to form blunt ends Second strand synthesis in site-directed mutagenesis Probe labeling using replacement synthesis Cloning of PCR products without reliance on ligation reactions. Always verify compatibility with your specific template, buffer, and downstream workflow.
One unit (U) is defined as the amount of enzyme that catalyzes the incorporation of 10 nmol of deoxyribonucleotide into acid-insoluble material in 30 min under standard assay conditions.
This enzyme is produced as Recombinant (E. coli) and supplied as a Research Use Only (RUO) reagent. Each lot is subjected to activity assay, purity assessment by SDS-PAGE, and functional validation prior to release. A Certificate of Analysis (CoA) and Safety Data Sheet (SDS) are available on request.
This enzyme is produced recombinantly in E. coli under stringent quality controls and validated on multiple substrate types. Key performance parameters include specific activity, substrate specificity, cofactor requirements, optimal pH/temperature range, and compatibility with downstream assays. Refer to the product datasheet for validated lot-specific activity data and recommended reaction conditions.
Yeasen Biotechnology supports custom enzyme solutions across multiple service lines — from GMP-grade bulk supply to directed enzyme engineering. Contact BioHippo to discuss requirements and initiate a project inquiry.
▶ GMP-Grade & Bulk Supply
Select Yeasen enzymes are available in GMP grade, manufactured in an ISO 13485-certified UCF.ME™ ultra-clean molecular enzyme facility with FDA Drug Master File (DMF) support.
- GMP-grade release testing and CoA documentation
- ISO 13485-certified production facility
- Scalable from milligram to multi-gram quantities
- Consistent lot-to-lot activity specifications
▶ Glycerol-Free & Custom Formulation
Glycerol-free enzyme formats are available for applications requiring lyophilization compatibility, liquid handling automation, or direct IVD master mix integration.
- Glycerol-free liquid format (standard and custom buffers)
- Lyophilization-ready enzyme preparation
- Custom reaction buffer optimization for specific assay conditions
- Compatible with freeze-drying workflows for point-of-care formats
▶ Molecular IVD RDC Service
Yeasen's Research and Development Contracting (RDC) team delivers end-to-end solutions for molecular diagnostic product development, covering enzyme selection through clinical validation support.
- Enzyme selection and performance matching
- Primer/probe design and reaction buffer optimization
- Sensitivity, specificity, and precision validation studies
- Stability studies and SNP evaluation
- Instrument platform compatibility assessment
▶ ZymeEditor™ Enzyme Engineering
Yeasen's proprietary ZymeEditor™ directed evolution and rational design platform enables the development of custom enzyme variants with tailored performance characteristics not available in off-the-shelf products.
- Directed evolution for enhanced thermostability, processivity, or fidelity
- Rational design for altered substrate specificity or cofactor requirements
- Library screening from Yeasen's proprietary enzyme variant collection
- Scale-up to commercial quantities upon candidate confirmation
ⓘ Customization services are fulfilled by Yeasen Biotechnology. Lead times and minimum order quantities vary by service type. Contact BioHippo for project scoping and pricing.
