| Field | Specification |
|---|---|
| Mfr No | |
| Product Type | |
| Shipping | |
| Source | Recombinant (E. coli) |
| Storage |
T4 RNA Ligase 2, also known as T4 RNA ligase 2, is an ATP-dependent double-stranded RNA ligase that exhibits both inter- and intra molecular ligation activities on RNA strands. Compared to T4 RNA Ligase 1, this enzyme displays a significantly higher activity in ligating nicks in double-stranded RNA than in ligating single-stranded RNA ends. The proximity of a 5'-phosphate group and a 3'-hydroxyl group is required for ligation. Moreover, it has the unique capability to ligate between the 3´-hydroxyl group of RNA and the 5´-phosphate group of DNA within a double-stranded structure.
Features
Strong ligation activity: capable of effectively linking dsRNA substrates.
No residual nuclease, nicking enzyme, or RNase.
Applications
Connecting the cleavage site of dsRNA.
Connecting the 3´ hydroxyl group of RNA to the 5´ phosphate group of double-stranded DNA structure.
Circular RNA preparation.
Specifications
|
Unit Definition |
One unit is defined as the amount of enzyme required to ligate 0.4 µg of an equimolar mix of a 23-mer and 17-mer RNAs in a total reaction volume of 20 µl in 30 minutes at 37oC |
|
Source |
Recombinant protein expressed by E. coli is derived from the Y10A gene of T4 bacteriophage |
|
Purity |
≥95% |
|
Reaction conditions |
React at 25oC for 1 hour |
|
Inactivation conditions |
React at 80oC for 5 min |
Glycerol Content |
Contains Glycerol |
Components
|
Components No. |
Name |
14652ES80 |
14652ES90 |
|
14652-A |
T4 RNA ligase 2 (10 U/μL) |
100 μL |
500 μL |
|
14652-B |
10×T4 RL2 Reaction Buffer |
500 μL |
1.5 mL |
Shipping and Storage
This product should be stored at -25 ~ -15oC for 1 years.
Figures
Figure 1. Verification of T4 RNA ligase 2 ligation efficiency.
Note: The 20 μL reaction system contains dsRNA ligation substrates at a final concentration of 20 μM.
Related Reading
Store this enzyme at -20°C and avoid repeated freeze-thaw cycles to preserve catalytic activity. The product is shipped Dry Ice and remains stable for up to one year from the date of manufacture when stored under recommended conditions. Aliquoting the stock solution into single-use volumes is recommended for enzymes used infrequently to minimize thermal cycling of the bulk stock.
Connecting the cleavage site of dsRNA. Connecting the 3 ´ hydroxyl group of RNA to the 5 ´ phosphate group of double-stranded DNA structure. Circular RNA preparation.. Always verify compatibility with your specific template, buffer, and downstream workflow.
One unit (U) is defined as the amount of enzyme that catalyzes the ligation of greater than 95% of HindIII-linearized lambda DNA fragments into high-molecular-weight concatemers in 30 min at 16°C in a 20 µL reaction volume.
This enzyme is produced as Recombinant (E. coli) and supplied as a Research Use Only (RUO) reagent. Each lot is subjected to activity assay, purity assessment by SDS-PAGE, and functional validation prior to release. A Certificate of Analysis (CoA) and Safety Data Sheet (SDS) are available on request.
Standard T4 DNA ligase requires overnight ligation at 16°C for optimal efficiency with cohesive ends. Quick Ligase formulations contain optimized buffer systems and enhanced enzyme concentrations that achieve >90% ligation efficiency within 5–15 minutes at room temperature, significantly accelerating cloning workflows without compromising junction fidelity.
Yeasen Biotechnology supports custom enzyme solutions across multiple service lines — from GMP-grade bulk supply to directed enzyme engineering. Contact BioHippo to discuss requirements and initiate a project inquiry.
▶ GMP-Grade & Bulk Supply
Select Yeasen enzymes are available in GMP grade, manufactured in an ISO 13485-certified UCF.ME™ ultra-clean molecular enzyme facility with FDA Drug Master File (DMF) support.
- GMP-grade release testing and CoA documentation
- ISO 13485-certified production facility
- Scalable from milligram to multi-gram quantities
- Consistent lot-to-lot activity specifications
▶ Glycerol-Free & Custom Formulation
Glycerol-free enzyme formats are available for applications requiring lyophilization compatibility, liquid handling automation, or direct IVD master mix integration.
- Glycerol-free liquid format (standard and custom buffers)
- Lyophilization-ready enzyme preparation
- Custom reaction buffer optimization for specific assay conditions
- Compatible with freeze-drying workflows for point-of-care formats
▶ Molecular IVD RDC Service
Yeasen's Research and Development Contracting (RDC) team delivers end-to-end solutions for molecular diagnostic product development, covering enzyme selection through clinical validation support.
- Enzyme selection and performance matching
- Primer/probe design and reaction buffer optimization
- Sensitivity, specificity, and precision validation studies
- Stability studies and SNP evaluation
- Instrument platform compatibility assessment
▶ ZymeEditor™ Enzyme Engineering
Yeasen's proprietary ZymeEditor™ directed evolution and rational design platform enables the development of custom enzyme variants with tailored performance characteristics not available in off-the-shelf products.
- Directed evolution for enhanced thermostability, processivity, or fidelity
- Rational design for altered substrate specificity or cofactor requirements
- Library screening from Yeasen's proprietary enzyme variant collection
- Scale-up to commercial quantities upon candidate confirmation
ⓘ Customization services are fulfilled by Yeasen Biotechnology. Lead times and minimum order quantities vary by service type. Contact BioHippo for project scoping and pricing.
