| Field | Specification |
|---|---|
| Mfr No | |
| Product Type | |
| Shipping | |
| Source | Recombinant (E. coli) |
| Storage |
T4 UvsX Recombinase, derived from the T4 bacteriophage, is a homolog of the RecA/Rad51 family and plays a critical role in the repair of double-strand DNA breaks and the restart of replication forks. This recombinase collaborates with other DNA-binding proteins or cofactors to form a nucleoprotein complex with single-stranded DNA (ssDNA). The complex identifies complementary regions on target DNA for hybridization, facilitating subsequent strand displacement reactions. The enzyme exhibits no nuclease activity.
Product Components
|
NO. |
Name |
11079ES60 (100 μg) |
11079ES03 (1 mg) |
11079ES08 (5 mg) |
11079ES20 (20 mg) |
|
11079 |
T4 UvsX protein (2 μg/μL) |
50 μL |
500 μL |
2.5 mL |
10 mL |
Product Applications
Primarily used in isothermal amplification techniques, such as Recombinase Polymerase Amplification (RPA).
Enzyme Storage Buffer Composition
50 mM Tris-HCl, 50 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 20% Glycerol, pH 7.5 at 25°C.
Shipping and Storage Conditions
Shipped with ice packs; store at -20°C. Shelf life: 2 years. Avoid repeated freeze-thaw cycles.
Precautions
1. The glycerol content in the T4 UvsX Recombinase storage buffer is 20%. It is recommended to aliquot the enzyme into separate portions to avoid repeated freeze-thaw cycles.
2. This product is intended for research use only.
3. For your safety and health, please wear a lab coat and disposable gloves during handling.
Store this enzyme at -20°C and avoid repeated freeze-thaw cycles to preserve catalytic activity. The product is shipped Dry Ice and remains stable for up to one year from the date of manufacture when stored under recommended conditions. Aliquoting the stock solution into single-use volumes is recommended for enzymes used infrequently to minimize thermal cycling of the bulk stock.
This enzyme is validated for use in Isothermal Amplification applications. It is suitable for use in both standard laboratory research settings and, where applicable, optimized reaction workflows requiring high specificity, sensitivity, or throughput.
Recombinase activity (µg/µL) is validated by strand exchange or recombination efficiency using defined oligonucleotide substrates under standard assay conditions.
This enzyme is produced as Recombinant (E. coli) and supplied as a Research Use Only (RUO) reagent. Each lot is subjected to activity assay, purity assessment by SDS-PAGE, and functional validation prior to release. A Certificate of Analysis (CoA) and Safety Data Sheet (SDS) are available on request.
UvsX recombinase (from T4 bacteriophage) coats single-stranded DNA primers and mediates strand invasion into homologous double-stranded DNA templates at 37–42°C. In RPA, UvsX works in concert with SSB protein (to stabilize displaced strands) and a strand-displacing polymerase to enable exponential, isothermal DNA amplification without thermocycling, making it highly suitable for point-of-care diagnostics.
Yeasen Biotechnology supports custom enzyme solutions across multiple service lines — from GMP-grade bulk supply to directed enzyme engineering. Contact BioHippo to discuss requirements and initiate a project inquiry.
▶ GMP-Grade & Bulk Supply
Select Yeasen enzymes are available in GMP grade, manufactured in an ISO 13485-certified UCF.ME™ ultra-clean molecular enzyme facility with FDA Drug Master File (DMF) support.
- GMP-grade release testing and CoA documentation
- ISO 13485-certified production facility
- Scalable from milligram to multi-gram quantities
- Consistent lot-to-lot activity specifications
▶ Glycerol-Free & Custom Formulation
Glycerol-free enzyme formats are available for applications requiring lyophilization compatibility, liquid handling automation, or direct IVD master mix integration.
- Glycerol-free liquid format (standard and custom buffers)
- Lyophilization-ready enzyme preparation
- Custom reaction buffer optimization for specific assay conditions
- Compatible with freeze-drying workflows for point-of-care formats
▶ Molecular IVD RDC Service
Yeasen's Research and Development Contracting (RDC) team delivers end-to-end solutions for molecular diagnostic product development, covering enzyme selection through clinical validation support.
- Enzyme selection and performance matching
- Primer/probe design and reaction buffer optimization
- Sensitivity, specificity, and precision validation studies
- Stability studies and SNP evaluation
- Instrument platform compatibility assessment
▶ ZymeEditor™ Enzyme Engineering
Yeasen's proprietary ZymeEditor™ directed evolution and rational design platform enables the development of custom enzyme variants with tailored performance characteristics not available in off-the-shelf products.
- Directed evolution for enhanced thermostability, processivity, or fidelity
- Rational design for altered substrate specificity or cofactor requirements
- Library screening from Yeasen's proprietary enzyme variant collection
- Scale-up to commercial quantities upon candidate confirmation
ⓘ Customization services are fulfilled by Yeasen Biotechnology. Lead times and minimum order quantities vary by service type. Contact BioHippo for project scoping and pricing.
