| Field | Specification |
|---|---|
| Mfr No | |
| Enzyme Type | |
| Expression System | |
| Form | Liquid |
| Formulation | |
| Product Type | |
| Purity | |
| Source | T7 Bacteriophage RNA Polymerase gene |
| Species | |
| Storage | |
| Target |
Product Description
T7 RNA Polymerase Recombinant
Overview
T7 RNA polymerase active enzyme synthesizes RNA at an increasing degree of that of E. coli RNA polymerase and it terminates transcription often. T7 RNA polymerase is very selective for initiation at its own promoter sequences and is resistant to antibiotics that inhibit E. coli RNA polymerase. In-vitro transcription of mRNA is achieved via bacteriophage T7 RNA polymerase using its ability to produce full-length RNA transcripts with high reliability, thoughT7 RNAP can manufacture as well immunostimulatory by products for example dsRNA which affect protein expression.
Specifications
| Purity | Greater than 95% as visualized by SDS-PAGE |
|---|---|
| Formulation | (500U/ul) in 50mM Tris-HCl (25°C, pH 7.9), 0.1M NaCl, 0.1mM EDTA, 2mM DTT, 0.1% Triton X-100 and 50% glycerol. |
| Storage | Two years when stored at -20°C, 2 weeks at 4°C. DO NOT STORE AT -70C. |
| Expression System | T7 Bacteriophage RNA Polymerase gene |
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