| Field | Specification |
|---|---|
| Mfr No | |
| Form | 2X Mastermix |
| Product Type | |
| Storage |
Your cost-effective choice for routine amplification!
Taq 2X PCR MasterMix is a ready-to-use MasterMix containing Taq DNA Polymerase (Cat. No. G009) in a uniquely-formulated buffer with gel loading dye. Taq is a highly thermostable enzyme that catalyzes the 5’-3’ synthesis of DNA and features 5’-3’ exonuclease activity, producing 3’-dA-tailed amplicons that are ideal for TA cloning vectors. While it lacks proofreading activity due to the absence of 3'-5' activity, Taq Polymerase is known for its robust performance and reliability in generating DNA products quickly and efficiently. Its ability to perform well at high temperatures makes it an excellent choice for routine PCR applications across various research needs.
Product Features:
- Consistent results over a wide range of DNA templates
- Excellent yield and sensitivity
- Contains gel loading dye for direct loading of PCR products onto an agarose gel
- Ideal for TA cloning vectors
- Included in abm's PCR Buffet Program
| Product Component | Quantity |
|---|---|
| Taq 2X PCR MasterMix | 400 rxn (10.0 ml) |
1 MasterMix contains 1.5 mM Mg2+
 |
ISO 13485:2016 MDSAP Certified |
| Component | Quantity |
|---|---|
| Taq 2X PCR MasterMix | 400 rxn (10.0 ml) |
| Specification | Value |
|---|---|
| Enzyme Type | PCR Mastermix |
| Format | 2X Mastermix |
| Hot Start | No |
| Fidelity | Standard |
| Mg²⁺ Concentration | 1.5 mM |
| Storage Conditions | Store at -20°C. |
The loading dye does not interfere with downstream cloning experiments. However, PCR clean-up is still advised prior to cloning due to the presence of excess primers, salts, and other buffer components that could potentially affect cloning efficiency.
This product is functionally equivalent to Cat. No. G013, G013-dye, G900, G900-dye (2X PCR Taq MasterMix with and without dye). Contact our customer service team [contact ABM technical support] for more information.
This product should be stored at -20°C to maintain its activity and stability. Ensure that the enzyme is kept in a frozen state until ready to use.
It is generally not recommended to store an assembled PCR reaction for long periods, as the activity of the polymerase can decrease over time. However, you can prepare the reaction mix and store it on ice for short periods (a few hours) before running the PCR. Always prepare fresh reactions when possible for the best results.
The enzyme is produced recombinantly in E. coli, which has been engineered to express the enzyme gene. While the original gene may come from another organism, all production and purification occur using E. coli under controlled conditions.
Yes. Products have 3′-dA tails, making them compatible with TA cloning vectors.
Use a touchdown PCR program for high-GC or complex targets. Reduce total volume to 25 µl for low-template reactions to improve efficiency.
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