| Field | Specification |
|---|---|
| Mfr No | |
| Product Type | |
| Shipping | |
| Source | Recombinant (E. coli) |
| Storage |
Taq DNA Ligase is a thermostable ligase that catalyzes the formation of a phosphodiester bond between the 5'-phosphate and 3'-hydroxyl groups of two adjacent oligonucleotides hybridized to the same complementary target DNA strand. This catalytic reaction occurs only when the two oligonucleotides are perfectly paired with the complementary target DNA and there is no gap between the two oligonucleotides. Therefore, it can be used to detect single-base substitutions. Taq DNA Ligase uses NAD+ as a cofactor and is active in the temperature range of 45°C to 65°C.
Applications
Specific detection of alleles using ligase detection reaction and ligase chain reaction
Mutagenesis by PCR amplification incorporating phosphorylated oligonucleotides
Homologous recombination
Specifications
|
Concentration |
40 U/μL |
|
Source |
Recombinant E. coli strain containing the ligase gene cloned from Thermus aquaticus HB8. |
|
Unit Definition |
One unit of activity is defined as the amount of enzyme required to ligate 50% of 1 µg of λDNA fragments digested with BstEII (12 bp sticky ends) within 15 minutes at 45°C in a 50 µL reaction volume |
|
Inactivation conditions |
Incubate at 45°C for 15 minutes. Add stop dye (50% glycerol, 50 mM EDTA, and bromophenol blue) to terminate the reaction |
Glycerol Content |
Contains Glycerol |
Components
|
Components No. |
Name |
11051ES80 |
11051ES84 |
11051ES92 |
|
11051-A |
Taq DNA Ligase (40 U/µL) |
50 μL |
5×50 μL |
|
|
11051-B |
10×Taq DNA Ligase Buffer（NAD+） |
250 μL |
500 μL |
5×500 μL |
Shipping and Storage
This product should be stored at -25 ~ -15℃ for 1 year.
Store this enzyme at -20°C and avoid repeated freeze-thaw cycles to preserve catalytic activity. The product is shipped Dry Ice and remains stable for up to one year from the date of manufacture when stored under recommended conditions. Aliquoting the stock solution into single-use volumes is recommended for enzymes used infrequently to minimize thermal cycling of the bulk stock.
Specific detection of alleles using ligase detection reaction and ligase chain reaction Mutagenesis by PCR amplification incorporating phosphorylated oligonucleotides Homologous recombination. Always verify compatibility with your specific template, buffer, and downstream workflow.
One unit (U) is defined as the amount of enzyme that catalyzes the ligation of greater than 95% of HindIII-linearized lambda DNA fragments into high-molecular-weight concatemers in 30 min at 16°C in a 20 µL reaction volume.
This enzyme is produced as Recombinant (E. coli) and supplied as a Research Use Only (RUO) reagent. Each lot is subjected to activity assay, purity assessment by SDS-PAGE, and functional validation prior to release. A Certificate of Analysis (CoA) and Safety Data Sheet (SDS) are available on request.
Standard T4 DNA ligase requires overnight ligation at 16°C for optimal efficiency with cohesive ends. Quick Ligase formulations contain optimized buffer systems and enhanced enzyme concentrations that achieve >90% ligation efficiency within 5–15 minutes at room temperature, significantly accelerating cloning workflows without compromising junction fidelity.
Yeasen Biotechnology supports custom enzyme solutions across multiple service lines — from GMP-grade bulk supply to directed enzyme engineering. Contact BioHippo to discuss requirements and initiate a project inquiry.
▶ GMP-Grade & Bulk Supply
Select Yeasen enzymes are available in GMP grade, manufactured in an ISO 13485-certified UCF.ME™ ultra-clean molecular enzyme facility with FDA Drug Master File (DMF) support.
- GMP-grade release testing and CoA documentation
- ISO 13485-certified production facility
- Scalable from milligram to multi-gram quantities
- Consistent lot-to-lot activity specifications
▶ Glycerol-Free & Custom Formulation
Glycerol-free enzyme formats are available for applications requiring lyophilization compatibility, liquid handling automation, or direct IVD master mix integration.
- Glycerol-free liquid format (standard and custom buffers)
- Lyophilization-ready enzyme preparation
- Custom reaction buffer optimization for specific assay conditions
- Compatible with freeze-drying workflows for point-of-care formats
▶ Molecular IVD RDC Service
Yeasen's Research and Development Contracting (RDC) team delivers end-to-end solutions for molecular diagnostic product development, covering enzyme selection through clinical validation support.
- Enzyme selection and performance matching
- Primer/probe design and reaction buffer optimization
- Sensitivity, specificity, and precision validation studies
- Stability studies and SNP evaluation
- Instrument platform compatibility assessment
▶ ZymeEditor™ Enzyme Engineering
Yeasen's proprietary ZymeEditor™ directed evolution and rational design platform enables the development of custom enzyme variants with tailored performance characteristics not available in off-the-shelf products.
- Directed evolution for enhanced thermostability, processivity, or fidelity
- Rational design for altered substrate specificity or cofactor requirements
- Library screening from Yeasen's proprietary enzyme variant collection
- Scale-up to commercial quantities upon candidate confirmation
ⓘ Customization services are fulfilled by Yeasen Biotechnology. Lead times and minimum order quantities vary by service type. Contact BioHippo for project scoping and pricing.
