| Field | Specification |
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| Mfr No | |
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Your cost-effective choice for routine amplification!
Taq DNA Polymerase is a highly thermostable enzyme designed for efficient DNA amplification in PCR applications. It catalyzes the 5’-3’ synthesis of DNA and features 5’-3’ exonuclease activity, producing 3’-dA-tailed amplicons ideal for TA cloning vectors. While it lacks proofreading activity due to the absence of 3'-5' activity, Taq Polymerase is known for its robust performance and reliability in generating DNA products quickly and efficiently. Its ability to perform well at high temperatures makes it an excellent choice for routine PCR applications across various research needs.
Product Features:
- Consistent results over a wide range of DNA templates
- Excellent yield and sensitivity
- Ideal for TA cloning vectors
- Ready-to-use mastermix is also available
- Included in abm's PCR Buffet Program
| Product Component | Quantity |
|---|---|
| Taq DNA Polymerase | 200 rxn (200 µl) |
| 5X Taq Buffer1 | 2 x 1.0 ml |
1 Buffer contains 1.5 mm Mg2+
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ISO 13485:2016 MDSAP Certified |
| Specification | Value |
|---|---|
| Enzyme Type | DNA Polymerase |
| Format | Enzyme Only |
| Hot Start | No |
| Fidelity | Standard |
| Mg²⁺ Concentration | 1.5 mM |
| Storage Conditions | Store at -20°C. |
It doesn't leave a poly-A tail but it does leave an "dA" overhang at the 3' ends.
This product is functionally equivalent to Cat. No. G008 and G126 (Taq DNA Polymerase). Contact our customer service team [contact ABM technical support] for more information.
This product should be stored at -20°C to maintain its activity and stability. Ensure that the enzyme is kept in a frozen state until ready to use.
It is generally not recommended to store an assembled PCR reaction for long periods, as the activity of the polymerase can decrease over time. However, you can prepare the reaction mix and store it on ice for short periods (a few hours) before running the PCR. Always prepare fresh reactions when possible for the best results.
The enzyme is produced recombinantly in E. coli, which has been engineered to express the enzyme gene. While the original gene may come from another organism, all production and purification occur using E. coli under controlled conditions.
Yes — the supplied 5X Taq Buffer contains 1.5 mM Mg²⁺ (final concentration when diluted).
Yes. Products have 3′-dA tails, making them compatible with TA cloning vectors.
Use a touchdown PCR program for high-GC or complex targets. Reduce total volume to 25 µl for low-template reactions to improve efficiency.
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de Cassia-Pires, R., de Melo, M. D. F. A. D., Barbosa, R. D. H., & Roque, A. L. R. (2017). Multiplex PCR as a tool for the diagnosis of Leishmania spp. kDNA and the gapdh housekeeping gene of mammal hosts. PLoS One, 12(3), e0173922. https://doi.org/10.1371/journal.pone.0173922
Ertabaklar, H., Malatyali, E., Özbay, E. P. Ö., Yildiz, I., Sinecen, M., Ertuğ, S., ... & Güçlü, Ö. (2021). Microsatellite-based genotyping, analysis of population structure, presence of Trichomonas vaginalis virus (TVV) and Mycoplasma hominis in T. vaginalis isolates from southwest of Turkey. Iranian Journal of Parasitology, 16(1), 81. https://doi.org/10.18502/ijpa.v16i1.5515
Jafar, S., Anjum, K.M., Zahoor, M.Y. et al. Development and validation of a universal primer pair for the taxonomic and phylogenetic studies of vertebrates. Mol Biol Rep 51, 332 (2024). https://doi.org/10.1007/s11033-023-09175-w
Liu, Q., Guo, X., Xun, G., Li, Z., Chong, Y., Yang, L., ... & Feng, Y. (2021). Argonaute integrated single-tube PCR system enables supersensitive detection of rare mutations. Nucleic acids research, 49(13), e75-e75. https://doi.org/10.1093/nar/gkab274
Prykhozhij, S. V., Ban, K., Brown, Z. L., Kobar, K., Wajnberg, G., Fuller, C., ... & Berman, J. N. (2024). miR-34a is a tumor suppressor in zebrafish and its expression levels impact metabolism, hematopoiesis and DNA damage. PLoS Genetics, 20(5), e1011290. https://doi.org/10.1371/journal.pgen.1011290
