tBuBHQ

Product details

• Chemical name: 2,5-Ditert-butylbenzene-1,4-diol.
• Also known as: DBHQ
• CAS number: 88-58-4
• Molecular formula: C14H22O2.
• Purity: >97%
• Concentration: 1-10 μM.
• Form: Lyophilized powder.
• Solubility: Ethanol or DMSO. Centrifuge all product preparations before use (10000 x g 5 min).
• Reconstitution: Ethanol or DMSO. Centrifuge all product preparations before use (10000 x g 5 min).
• Shipping: Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
• Target: SERCA
• Source: Synthetic
• Activity: tBuBHQ is an inhibitor of the endoplasmic reticulum Ca2+-ATPase1.
• Alternative names: DBHQ

Scientific background

tBuBHQ is a chemically synthesized compound1. It is widely used as a potent selective inhibitor of the sarcoplasmic reticulum Ca2+ ATPase pump (SERCA). The pump transfers Ca2+ from the cell cytosol to the endoplasmic reticulum lumen during muscle relaxation. Inhibition of SERCA by tBuBHQ causes a dose-dependent, sustained, and marked increase of cytoplasmatic Ca2+ and store-operated Ca2+ depletion1. tBuBHQ also causes superoxide anion production independently of Ca2+ secretion2.

Lead time: 1-2 Business Days

Country of origin: Israel/IL

Applications key

Application key: FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay.

• SDS (PDF)

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