| Field | Specification |
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| Mfr No | |
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The TCCSUP cell line was established from a Grade IV transitional cell carcinoma (TCC). The cell line was derived from a highly anaplastic carcinoma with characteristics of aggressive malignancy, including rapid proliferation and poor differentiation. Cytogenetic analysis revealed an abnormal karyotype with a lack of a clear modal number, and distinct marker chromosomes were observed throughout its in vitro passages. Morphologically, TCCSUP cells display epithelial-like and fibroblast-like features, consistent with the heterogeneity of aggressive TCC tumors.
In vitro, TCCSUP cells exhibit robust growth in monolayer cultures. The cell line has been extensively used in cancer research, particularly in studies of bladder cancer biology and therapeutic response. Notably, TCCSUP cells retain tumor-associated antigens, making them a valuable model for immunological studies and for developing antigen-targeting therapies.
Further molecular characterization has highlighted its utility in high-throughput drug screening and genetic studies. TCCSUP cells have been included in large-scale proteomic and genomic analyses, including reverse-phase protein array studies, revealing alterations in signaling pathways such as PI3K/AKT and MAPK. These findings align with the cell line's tumorigenic properties and its relevance as a model for understanding the molecular underpinnings of bladder cancer progression.
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- cultureMedium: EMEM (MEM Eagle), w: 2 mM L-Glutamine, w: 2.2 g/L NaHCO3, w: EBSS (Cytion article number 820100a)
- supplements: Supplement the medium with 10% FBS and 1% NEAA
- dissociationReagent: Accutase
- doublingTime: 30 to 40 hours
- subculturing: Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
- fluidRenewal: 2 to 3 times per week
- freezeMedium: As a cryopreservation medium, use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
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